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Annealing
Antisense Oligos
Beacons or DLPs
Cloning
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Most Popular Articles...
Is it OK to use purified water instead of buffer to resuspend my oligos? I'm worried about the low pH of the water causing depurination of the DNA. Is this a legitimate concern?
modified: 4/5/2005
I want resuspend the oligos. What do I do?
modified: 4/19/2005
Whats the best way to mix the oligos with the TE buffer? Should I vortex them and spin them down. Or will flicking the tube on the bottom a couple of times work?
modified: 9/20/2007
Can you tell me the minimum number of bases that can be annealed?
modified: 1/6/2006
I was using a desalted 27mer to amplify a gene which I then cloned and found a deletion in the primer sequence. Is this common?
modified: 4/17/2006
Latest Additions...
are there any ready made endogenous primers of GAPDH or BEta -actin for mice.
modified: 5/14/2008
I would like to know if you offer the synthesis of oligos containing abasic sites (tetrahydrofuran) for autosticky PCR.
modified: 5/14/2008
Can your miRNA Starfire kit can be used for biotin end-labeling of RNA? If so, can it only label 5' ends, or do you have a method of labeling 3' ends?
modified: 5/14/2008
When I order a miRNA starfire kit, should i supply my specific target domain, so you can synthesis a specific template oligo for me? or, i can just use the universal template you supplied?
modified: 5/14/2008
We are using radioactive dCTP i labelling reactions. Do you have plan to develop StaFire kit using dCTP as radioactivity?
modified: 5/14/2008
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