Cited
Scientific Publications Referencing IDT Products

CRISPR + Gibson Assembly® Approach Addresses Cloning Limitations

A method for combining CRISPR/Cas9 genome editing and the Gibson Assembly® Method to seamlessly assemble large DNA constructs.

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DNA Mutations and Copy Number Alterations Identified in FFPE Tumor Samples Suggest Potential Therapeutic Targets

Next generation sequencing was used to identify DNA mutations and copy number alterations in FFPE phyllodes tumor samples. Results were validated with Sanger sequencing and IDT PrimeTime® qPCR Assays.

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Nano-Delivery of DsiRNAs Results in Improved Gene Silencing and Anticancer Activity

Dendrimer-based, targeted nano-delivery system Dicer Substrate RNAs (DsiRNAs) leads to improved gene silencing and anticancer activity in prostate cancer models in vitro and in vivo.

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Overlapping Biocontainment Strategies for Genetically Modified E. Coli.

gBlocks Gene Fragments are used to create codon optimized components of a biocontainment system for genetically modified E. Coli.

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Validating Sequencing Results of a Repetitive Element With Digital Droplet PCR

Mobilization of long interspersed element 1 (L1) can be involved in human disease and cancer. Read how the specificity and sensitivity of digital droplet PCR can be used to validate the detection of rare L1 insertion events.

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Advantageous Properties of Dicer-Substrate Interfering RNA (DsiRNA) Enable Improved RNAi-Based Gene Silencing

Comparison of cononical siRNA design to DsiRNAs for performance in siRNA processing and RISC assembly, leading to stronger silencing efficacy.

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Double-Quenched Probes Increase Sensitivity of qPCR Assay Detecting Viral Load

Use of a ZEN™ Double-Quenched Probe results in a marked decrease in background fluorescence compared to an identical TaqMan® probe containing only a single quencher. The data suggest that such double-quenched probes may be a better approach for other qPCR probe-based assays.

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Decoding Cas9 Orthologs Using gBlocks® Gene Fragments

gBlocks Gene Fragments were used to create Cas9 orthologs as well as tracRNA expression cassettes for each ortholog tested.

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Nanoparticle Delivery of TNF-α targeting DsiRNA as Treatment for Rheumatoid Arthritis

DsiRNA-loaded PLGA nanoparticles mediate dose-dependent silencing of TNF-α in vitro.

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A Fast, Sensitive, Cost-Effective Alternative to Radiolabeling and HPLC/MS for Measuring dNTPs

A rapid and sensitive fluorescence-based method that uses synthetic templates (IDT oligonucleotides), a PrimeTime® Primer, and ZEN™ Double-Quenched Probes for quantifying cellular dNTPs.

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Caffeine Addicted Bacteria

Use of IDT gBlocks® Gene Fragments in Gibson Assembly® reactions to generate some of the plasmid constructs in this work.

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Barcoding Living Cell Populations with Zinc-Finger DNA-Binding Domains

IDT gBlocks® Gene Fragments were used to generate surface zinc-finger DNA-binding domains for barcoding distinct cell lines. These live cells could then be tracked within a heterogeneous mix of cells.

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Mimicking Heredity and Evolution using Xeno-Nucleic Acid Aptamers

The researchers engineered polymerases that can synthesize XNA from a DNA template and reverse transcribe XNA back into DNA. Results showed high affinity and specificity of target binding, demonstrating the capacity of XNAs for Darwinian evolution.

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Mutagenesis Using gBlocks® Gene Fragments

Just 3 synthetic, high fidelity, double-stranded gBlocks™ Gene Fragments used to mutate 18 different sites over the entire exon 7, 1039 bp sequence.

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Quick Oligonucleotide Assembly

A protocol for assembling high-fidelity Ultramer™ oligonucleotides with overlapping sequences into large constructs using Saccharomyces cerevisiae.

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Efficient Multiplex Processing of Pooled Barcoded Samples

Authors describe a cost-effective procedure for parallel genomic library preparation from multiple samples followed by targeted enrichment of pooled barcoded samples. The protocol is compatible with microarray- and solution-based capture methods.

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