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Sequence # Bases        
Target Type  
Oligo Conc µM  
Na+ Conc  mM  
Mg++ Conc mM  
dNTPs Conc mM  
Default Settings
    
Analyze
       
Hairpin
Self-Dimer
Hetero-Dimer
NCBI Blast
TM Mismatch
LNA Conversion
 5'-  -3'
Clear Sequence
   
Add To Order

Results 5' mods Internal Mods 3' mods Mixed Bases
Standard Mixed Base Instructions
To use a Standard Mixed Base, simply type in the IUB symbol (from the table below) which represents the desired mix.

R
 A,G
Y
 C,T
M
 A,C
K
 G,T
S
 C,G
W
 A,T
H
 A,C,T
B
 C,G,T
V
 A,C,G
D
 A,G,T
N
 A,C,G,T
Custom Mixed Base Instructions
To use Customer Mixed Bases
  1. Enter the desired percentage of each base(Integers Only, Totalling 99% or 100%).
  2. Press 'Use Mix Base' button to add your custom mixed base.
Please note: There is an additional charge for hand mixing these custom bases.
 
 %A%C%G%TName (optional)
 
Use Mixed Base
Base Notations
  1. DNA = A, C, G, T and U, I
  2. Mixed Bases = Please enter bases in UPPERCASE
  3. Phosphorothioated DNA = A*, G*, C*, T*
  4. RNA = rA, rG, rC, rU
  5. Phosphorothioated RNA = rA*, rG*, rC*, rU*
  6. 2'O-Methyl RNA = mA, mG, mC, mU
  7. Phosphorothioated 2'O-Methyl RNA = mA*, mG*, mC*, mU*
  8. Locked Nucleic Acid (LNA) = +A, +G, +C, +T
 
 
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