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Sequence # Bases  
Target Type  
Oligo Conc µM  
Na+ Conc  mM  
Mg++ Conc mM  
dNTPs Conc mM  
  
 5'- -3'

 

Base Notations
DNA = A, C, G, T and U, I
Mixed Bases = Please enter bases in UPPERCASE
Phosphorothioated DNA = A*, G*, C*, T*
RNA = rA, rG, rC, rU
Phosphorothioated RNA = rA*, rG*, rC*, rU*
2'O-Methyl RNA = mA, mG, mC, mU
Phosphorothioated 2'O-Methyl RNA = mA*, mG*, mC*, mU*
Locked Nucleic Acid (LNA) = +A, +G, +C, +T
Standard MixBase Definitions
R A, G
Y C, T
M A, C
K G, T
S C, G
W A, T
H A, C, T
B C, G, T
V A, C, G
D A, G, T
N A, C, G, T