RNaseAlert^TM Substrate and Nuclease Detection System

Single-stranded RNases are ubiquitous, hard to eliminate and can rapidly degrade important samples used in microarray studies, real-time PCR, Northern blots or cDNA cloning. IDT has developed reagents that allow for rapid, sensitive detection of RNases and DNases (click here for DNaseAlert^TM). These reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescent signal only after nuclease degradation. The assay can be read visually or measured and quantified using fluorometry. Assays can be used qualitatively to test lab reagents, equipment and supplies for nuclease contamination. Assays can also be used quantitatively to study enzyme kinetics.

The RNaseAlert^TM substrate employs a FAM^TM (fluorescein) reporter (Em 520 nm) and a dark quencher. The RNA sequence has been carefully optimized to react with a wide variety of ribonucleases. Intact, the substrate has little or no fluorescence. When cleaved by an RNase, the substrate fluoresces green (490 nm or UV excitation, 520 nm emission).

RNaseAlert^TM is available in single-use tubes which are ideal for rapid hand-held visual assays at the research bench, or as bulk substrate which can be used in either visual assays or read in a fluorometer. RNaseAlert^TM is also available as a kit that provides 25 single-use tubes of the RNaseAlert^TM Substrate. The kit also includes a buffer optimized for detection of a wide variety of RNases, nuclease free water, RNase A (for use as a positive control), and 50 ml of Nuclease Decontamination Solution (to clean lab surfaces found to have nuclease contamination).

RNaseAlert^TM Kit Components:
25 50 pmole Fluorescent Substrate tubes
250 µl RNaseAlert^TM Buffer
2 ml Nuclease-Free water
50 µl RNase A
50 ml Nuclease Decontamination Solution
Kit quality guaranteed for six months from date received when stored at -20°C.

RNaseAlert^TM

Enlarged View Examination of RNase-free sample versus RNase positive control.

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