Most Popular Articles... Is it OK to use purified water instead of buffer to resuspend my oligos? I'm worried about the low pH of the water causing depurination of the DNA. Is this a legitimate concern? modified: 4/5/2005 I want resuspend the oligos. What do I do? modified: 4/19/2005 Whats the best way to mix the oligos with the TE buffer? Should I vortex them and spin them down. Or will flicking the tube on the bottom a couple of times work? modified: 9/20/2007 Can you tell me the minimum number of bases that can be annealed? modified: 1/6/2006 I was using a desalted 27mer to amplify a gene which I then cloned and found a deletion in the primer sequence. Is this common? modified: 4/17/2006

Latest Additions... Does your gene synthesis service also include subcloning into customer specified vector? modified: 3/3/2008 I am ordering IDT oligos from Brazil (via Prodimol, your local distributor) and I was informed about the "ultramers" method for oligo purification. Is this purification good enough for cloning ? My oligos are 64 and 67 bp long and I will clone them into a vector for shRNA expression. Thanks modified: 12/26/2007 I am looking for the very short size standard marker that is biotinylated. Could you biotinylate the your 10/60 Ladder and 20/100 Ladder? modified: 12/26/2007 Do the positive and negative controls in the TriFecta Kit come with all primers and RT-PCR materials or do they need to be purchased separtely? modified: 11/6/2007 Which type of purification should I choose? modified: 10/30/2007

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