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Should I amplify my gBlocks® Gene Fragments when I receive them?

gBlocks Gene Fragments are normalized to 200 ng and delivered dried down, which should provide sufficient DNA to perform most cloning protocols 2–4 times. We rec­ommend avoiding amplification of gBlocks Gene Fragments because of the potential for polymerase introduced errors. If you need to amplify your gBlocks Gene Fragments or require more material than the 200 ng provided, it is strongly recommended that you use a high-fidelity polymerase in order to limit the introduction of errors into your gBlocks Gene Fragments sequence. For additional information, download a copy of the gBlocks Gene Fragments user guide or contact us at genes@idtdna.com.