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How can I calculate the Tm of an oligo that incorporates mismatches?

The free, IDT online OligoAnalyzer® tool (www.idtdna.com/scitools) allows research­ers to set mismatches and then calculate Tm. Enter your sequence into the Input box, and then click the Tm Mismatch button. Click on the base you wish to change and then set the mismatch base using the drop down menu. Click on the Calculate button, and the exact match and the mismatch Tm will be displayed.