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What modifications does IDT offer for click chemistry?

Alkynes readily react with azides in the presence of copper to form stable triazole bonds, the basis for click chemistry. IDT offers several modifications used in click chemistry:

  • Hexynyl is used to introduce a 5′ terminal alkyne group.

  • 5-Octadiynyl dU is a modified base with an 8 carbon linker terminating in an alkyne group. This modified base is preferred when inserting alkynes at internal positions within a sequence.

  • Azide NHS Ester leaves a free azide group available to click with a free alkyne.

  • Also inquire about using a 3’Alkyne Modifier, as well as DBCO used for copper-free click chemistry.

Read more about click chemistry in the DECODED article, Oligo Modification—Post-Synthesis Conjugation Explained.