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Ask Alex
Your Questions For Our Resident Expert

What does sequence-verified mean for gBlocks® Gene Fragments?

gBlocks Gene Fragments are double-stranded DNA fragments that are ideal for use in synthetic biology, gene construction and traditional cloning applications.

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Why does the Tm on my spec sheet differ from that generated by the IDT SciTools® programs (e.g., RealTime PCR, OligoAnalyzer® tools)?

The IDT RealTime PCR design tool calculates Tm under real-time PCR conditions different from those on the spec sheet.

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Why should I sequence multiple colonies when cloning?

IDT recommends sequencing at least 8–10 separate colonies to get a realistic representation of the clonal population and to provide a good chance of finding the correct sequence if one exists.

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How can I review my order history, and how do I update it if I move?

To review your order history, go to the “Order” tab on the IDT website (www.idtdna.com) and select “Order History”.

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How do I resuspend my PrimeTime® qPCR Assay?

PrimeTime qPCR Assays can be re-suspended as 40X, 20X, or 10X stocks.

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