Your Questions For Our Resident Expert
Getting the most accurate Tm estimation.
For most applications, it is best to purify PCR products by gel electrophoresis.
The scale of the oligonucleotide synthesis reaction is based solely on the starting material. As no chemical reaction is 100% efficient, the final deliverable amount is never equal to the starting amount for synthesis.
Labeled probes should, for the most part, be handled in the same fashion as other custom oligonucleotide sequences. We recommend resuspending probes in TE buffer.