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Your Questions For Our Resident Expert

How do I enter RNA sequences online?

Oligonucleotides containing at least one RNA base must be ordered under “Custom RNA Oligos” from the order main menu page.

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When is it important to get HPLC purification?

HPLC purification helps remove truncated synthesis products and enrich purity. Purification is recommended for oligos greater than 40 bases in length and for many modified oligos.

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What is your protocol for annealing oligos?

It is sometimes necessary to make double-stranded DNA from single-stranded oligos. While the annealing procedure is simple, attention to a few details can greatly reduce the presence of undesired single-stranded material.

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What is the minimum number of bases that can be annealed?

You can anneal an oligo of any size to its target, but longer sequences lead to more stable duplexes.

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Do synthetic oligonucleotides contain a 5’ phosphate?

The 5’ end of an unmodified synthetic oligo terminates in a hydroxyl group.

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