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Your Questions For Our Resident Expert

How do I order modified oligos online?

Modified oligos can be ordered from our Custom DNA Synthesis or Custom RNA Synthesis page.

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What are OligoCard payment cards?

OligoCard payment cards are prepaid cards that can be used to purchase IDT products.

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Where can oligo invoices, quality control documents, and specification sheets be found online?

For orders placed via the web, all of these documents are uploaded to your Order History.

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How many reactions will I be able to do with 25 nmole PCR primers?

Most PCR reactions use 0.1−0.5 μM primer. Assuming a maximum concentration of 0.5 μM and a reaction volume of 20 μL, each reaction will require 10 pmoles of oligonucleotide primer.

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What secondary structure considerations need to be included when designing primers for PCR?

When designing primers for PCR, two types of secondary structures should be analyzed: dimers and hairpins. For dimers, both self- and hetero-dimers should be reviewed.

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