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Special, Online Only Articles from IDT

A novel, high-fidelity Cas9 improves CRISPR editing accuracy without sacrificing performance

Webinar summary: Interested in reducing Cas9 off-target editing events? A high-fidelity Cas9 can provide more accurate CRISPR genome editing.

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Improve the specificity of SNP genotyping

Webinar summary: This novel, simple genotyping method uses blocked, cleavable primers and RNase H2 to prevent off-target and primer-dimer interactions. Learn how these single-tube reactions, easily run in high-throughput mode, can improve the accuracy and sensitivity of your genotyping experiments.

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A solution for sample cross-talk and index hopping in multiplexed NGS

Webinar summary: Learn about the steps in NGS sample preparation that can lead to index hopping and sample crosstalk. Dr Kristina Giorda provides expert advice on how to reduce these artifacts at each point, providing cleaner, demultiplexed NGS data.

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Analyzing the exome—focus your NGS analysis with high-performance target capture

Webinar review: Targeted sequencing capture, using probe pools or panels, can increase read depth and the number of samples per run, while decreasing sequencing cost and simplifying data analysis. See how using individually synthesized, quality-checked, DNA target capture probes (xGen Lockdown Probes) covering the human exome (xGen Exome Research Panel) perform across a variety of metrics and how the xGen Exome Research Panel compares to other available exome panels.

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NGS detection of low frequency genetic variants using novel, molecular sequencing adapters

Webinar review: Watch this webinar recording to learn about unique molecular adaptors and a high-performance target capture method for NGS analysis of low frequency variants.

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Using CRISPR genome editing for gene knockout and homology-directed repair (HDR)

Webinar review: Watch our webinar recording for expert guidance on a complete CRISPR genome editing workflow, including available tools and protocols. Also, see what we have learned about homology-directed repair and a new option for repair templates.

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CRISPR-Cpf1 expands genome editing to new target sites

Webinar summary: Learn how the Alt-R® CRISPR-Cpf1 System can be an effective alternative to CRISPR-Cas9 genome editing. See how Cpf1 compares to Cas9 for editing efficiency, and what is the best method for delivering the RNA and protein components to your cells.

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NGS target capture recommendations for FFPE samples

Webinar review: Learn how it is possible to create high quality target capture libraries from formalin-fixed, paraffin-embedded samples. Dr Kristina Giorda presents an FFPE sample workflow with a concise explanation of DNA quality analysis and how quality assessment can be used to guide the amount of DNA input for NGS library preparation.

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iGEM, and the vision for the next generation of synthetic biology researchers

Randy Rettberg, Co-founder and President of iGEM, discusses the 2016 iGEM competition in this video interview. Learn about his vision for the future for the students that participate in iGEM, as well as the future of synthetic biology.

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Improve probe-based qPCR experiments

Webinar summary: Learn about the benefits of our PrimeTime® Gene Expression Master Mix for qPCR experiments. Suitable for singleplex and multiplex experiments, online tools for planning qPCR experiments, and a discussion of the excellent thermal stability of the master mix.

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Strategies for optimizing high throughput qPCR for expression profiling

Webinar summary: Learn how to address the challenges of high throughput RT-qPCR expression profiling from a prominent qPCR expert, Dr. Mikael Kubista (TATAA Biocenter, Sweden).

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Webinar: Alt-R® CRISPR-Cas9 System ribonucleoprotein delivery optimization

Webinar: Genome editing using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) provides excellent editing efficiency, while reducing off-target editing and cell death. Watch this recorded presentation to find out how to easily generate and deliver CRISPR RNAs and Cas9 nuclease in an RNP format, using the optimized Alt-R® CRISPR-Cas9 System.

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Melt curve analysis for improved intercalating dye qPCR

Listen to this discussion of the benefits and limitations of melt-curve analysis using clear example—presented as a webinar. Read how PrimeTime Predesigned qPCR Primer Assays are designed to be compatible with intercalating dyes, and can also be transitioned to probe-based assays.

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Advantages of high quality, probe-based gene capture panels

Target enrichment by hybrid capture lets you focus your genomic analysis on specific regions of interest, increasing depth of coverage of targeted sequences and improving the detection of rare genomic events. You can create custom human gene capture panels quickly and cost-effectively using IDT preconfigured pools of probes targeting the coding sequences (CDS) of human protein-coding genes, or with predesigned disease or exome panels.

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Nanoparticle encapsulation method delivers DsiRNA to difficult-to-transfect cells

Research profile: Precision NanoSystems Inc has developed unique reagents and equipment that enable the simple assembly of novel nanoparticles, resulting in greatly improved delivery of RNA payloads. Learn about use of products to deliver DsiRNAs, 27-mer siRNAs, into primary hippocampal neuron cells and neuronal cells in vivo.

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Augmenting pathology data with molecular profiling for improved cancer treatment

Research profile: Foundation Medicine uses a unique approach to targeted next-generation sequencing to generate molecular information that will better inform cancer categorization and treatment decisions. Review this summary and view the presentation given at the 2014 Association for Molecular Pathology Annual Meeting (AMP) by Dr Geoff Otto (Foundation Medicine).

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Improving uniform coverage of targeted sequences for NGS

Learn about the challenges researchers face for obtaining uniform coverage of NGS data and how IDT xGen® Lockdown® Probes are uniquely positioned to facilitate uniform sequence coverage.

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The gene construction revolution

See how use of high-quality, custom dsDNA fragments as a starting material allows you to turn what might otherwise be multi-step cloning assemblies into simpler reactions.You can often just order the entire target sequence ready for cloning or other uses.

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You want a unicorn with your oligos? Umm, yeah we can do that!

Injecting a little fantasy into your biological reality.

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