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Webinar: Alt-R® CRISPR-Cas9 System ribonucleoprotein delivery optimization

The CRISPR (clustered regularly interspaced palindromic repeat)/Cas9 genome editing method has many advantages over other genome editing techniques. CRISPR/Cas9 is much easier to implement than engineered nucleases, and the necessary components can be generated using a variety of standard molecular biology methods. However, the various CRISPR strategies perform differently, and choosing the wrong CRISPR reagents can lead to poor editing efficiency, cell toxicity, off-target editing, and increased labor and material costs for the lab.

IDT has developed a simple and affordable CRISPR-Cas9 solution that outperforms other CRISPR reagents. In this recorded webinar, Dr Rolf Turk provides an overview of the advantages of using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) complex in genome editing experiments, and explains why it is the most efficient driver for genome editing compared to alternative methods, such as expression plasmids or the use of sgRNAs. Significant attention is given to IDT protocols for RNP delivery, and how researchers can optimize transfection or electroporation methods for delivery of the RNP. Watch the recorded webinar below to find out how the Alt-R® CRISPR-Cas9 System and RNP delivery can simplify and improve your genome editing research.

Product focus—genome editing with Alt-R® CRISPR Reagents

Alt-R CRISPR-Cas9 System

The Alt-R CRISPR-Cas9 System includes all the reagents needed for successful genome editing. Based on the natural S. pyogenes CRISPR-Cas9 system, the Alt-R CRISPR-Cas9 System offers numerous advantages over alternative methods:

  • Higher on-target potency than other CRISPR systems
  • Precision control with delivery of Cas9 ribonucleoprotein (RNP)
  • Efficient delivery of the RNP with lipofection or electroporation
  • No toxicity or innate immune response activation, in contrast to in vitro transcribed Cas9 mRNA and sgRNAs

Learn more about the Alt-R CRISPR-Cas9 System.

Alt-R CRISPR-Cpf1 System

The Alt-R CRISPR-Cpf1 System allows for new CRISPR target sites that are not available with the CRISPR-Cas9 System, and produces a staggered cut with a 5′ overhang. These reagents:

  • Enable genome editing in organisms with AT-rich genomes
  • Allow interrogation of additional genomic regions compared to Cas9
  • Require simply complexing the crRNA with the Cpf1 protein—no tracrRNA needed
  • Permit efficient delivery of the RNP into cells by electroporation

Learn more about the Alt-R CRISPR-Cpf1 System.

CRISPR support tools

Additional CRISPR reagents extend the ease-of-use and performance of the Alt-R system through options for fluorescent visualization, enhanced nuclease transfection, and genome editing detection.

Find out more about IDT’s entire line of CRISPR products.

Additional resources

CRISPR guide RNA format affects genome editing outcomes—Learn how use of different formats for the guide RNAs associated with CRISPR-Cas9 genome editing can lead to different editing outcomes. The optimized, short RNA oligos that make up the crRNA and tracrRNA components of the Alt-R® CRISPR-Cas9 System outperform other CRISPR guide RNA formats. Unlike DNA expression constructs, short RNA oligos are unable to incorporate into the target genome, resulting in cleaner editing.

6 pieces of data that will change how you set up your CRISPR-Cas9 experiments—Discover how you can improve the efficiency of CRISPR-Cas9 genome editing. IDT scientists evaluated several factors that influence how we design and perform genome editing experiments. Review the data and results for 5 important factors that were addressed. These experimental findings resulted in a set of potent CRISPR tools that are now offered as the Alt-R® CRISPR-Cas9 System.

Improve your genome editing with the Alt-R® S.p. Cas9 Nuclease 3NLS and modified crRNAs—Product Spotlight: Methods for CRISPR-Cas9 genome editing are diverse, but not all of them perform equally well. IDT now offers the Alt-R S.p. Cas9 Nuclease 3NLS that, when used in combination the optimized Alt-R CRISPR crRNA and tracrRNA, provides a highly effective editing solution that is also easy to use.

Getting started with Alt-R® CRISPR-Cas9 genome editing—Webinar: Watch a recording of this webinar to learn about the components of the Alt-R CRISPR-Cas9 System, get information on designing Alt-R CRISPR crRNA oligos, and review the genome editing protocol from the user guide.

Author: Author: Hans Packer, PhD, is a scientific writer at IDT.

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