There is often confusion surrounding the meanings of “synthesis scale” and “synthesis yield” in custom oligonucleotide manufacturing. Simply put, scale refers to the amount of starting material used to synthesize your oligonucleotide. It is composed solely of the 3‘-most nucleotide of a sequence attached to a solid support and housed within the column used to make the oligonucleotide.
Yield, on the other hand, refers to the amount of final product recovered after all of the synthesis and purification steps associated with the oligonucleotide have been completed. As no chemical reaction, even the coupling of simple DNA bases, is 100% efficient, the final deliverable amount of oligonucleotide is never equal to the starting amount for synthesis. Modifications, secondary structures, and purification services further affect the final delivery amount (yield).
IDT offers guaranteed yields based on all of these factors that define the minimum amount of oligonucleotide you will receive for a given construct. Once you have selected the synthesis scale, modifications, and purification services, check your shopping cart for the IDT minimum yield guarantee for that oligonucleotide order.
Author: Ellen Prediger, PhD is Director of Scientific Communication at IDT.