DECODED Newsletter Categories Your Research Core Concepts Competitive Edge Find Out First Pipet Tips Ask Alex Web Tools Product Spotlight Mixed Bases Calendar Did You Know? Cited Complementary Strands Archives Browse Issues: DECODED Online (Current) DECODED 4.2 qPCR (April 2014) DECODED 4.1 (January 2014) DECODED 3.4 (October 2013) DECODED 3.3 (July 2013) DECODED 3.2 (April 2013) DECODED 3.1 (January 2013) DECODED 2.4 (October 2012) DECODED 2.3 (July 2012) DECODED 2.2 (April 2012) DECODED 2.1 (January 2012) DECODED 1.3 (October 2011) DECODED 1.2 (July 2011) DECODED 1.1 (April 2011) Subscribe Find Out First New Products & Updates Print Page All posts More ZEN/Fluorophore Combos! Use ZEN Double-Quenched Probes for Multiplex qPCR ZEN Double-Quenched Probes increase sensitivity and provide improved precision in qPCR applications. Recent testing has shown that ZEN Double-Quenched Probes increased endpoint signal by more than 30% and decreased background fluorescence 3 fold when compared to traditional Black Hole or TAMRA™ quenchers. Researchers now have the ability to run multiplex qPCR experiments that take advantage of the low background and increased sensitivity that ZEN Double-Quenched Probes offer. Newly available PrimeTime qPCR Assays featuring ZEN Double-Quenched Probes with HEX and TET dyes, along with previously offered FAM assays, are compatible with a variety of real-time PCR instruments. The addition of JOE- and MAX™-labeled probes provides even more flexibility when designing experiments. For more about ZEN Double-Quenched Probes see the Product Spotlight article, Design qPCR Probes With high Tms Without Sacrificing Quenching Capacity.. Recommendations on dye selection for multiplex real-time PCR assays can be found on the IDT website, www.idtdna.com, under the Support menu.