The PrimerQuest design software—one of the most widely accessed tools on the IDT website—enables custom design scenarios as well as designs involving species other than human, mouse, and rat (IDT recommends using the PrimeTime® qPCR Assay Library Selection Tool for human, mouse, and rat qPCR assays).
With an upgrade of the design engine used by the PrimerQuest tool, even better qPCR assay designs are now possible. The highly flexible, updated PrimerQuest program allows you to customize designs for multiple scenarios. Design qPCR assays by selecting specific target regions. Alternatively, specify primers to find the best possible probe and vice versa. Since this program now directly links to NCBI databases, multiple formats of gene identification annotations are accepted.
With this recent update, the program now provides:
- 1-click design for most common situations
- Simplified parameters for custom design
- Batch mode for designing up to 50 primers simultaneously
- Option for delivery in tubes or plates
Access the PrimerQuest Program (red arrow) and PrimeTime qPCR Assay Library Selection Tool at www.idtdna.com/scitools. The PrimeTime qPCR Assay Library Selection Tool can be found under the qPCR menu.