Genome Editing
Support and Educational Content

A recombinant Cas9 enzyme that drastically reduces CRISPR off-target effects

Learn about the Cas9 variant, Alt-R® S.p. HiFi Cas9 Nuclease 3NLS, that greatly reduces off-target cutting events during CRISPR genome editing. At the same time, it maintains the high level of on-target editing efficiency as wild-type Cas9 nuclease.

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CRISPR-Cpf1, an alternative to Cas9 for targeting AT-rich genomes

Did you know that use of CRISPR endonuclease Cpf1 (also known as Cas12a) can greatly expand the number of target sites available for genome editing? Unlike the G-rich PAM requirement of Cas9, Cpf1 recognizes a T-rich PAM, TTTV. Not only is this enzyme useful for targeting AT-rich genomes, but it has applications in altering disease or phenotype-linked mutations in AT-rich regions through homology-directed repair. In addition, Cpf1 does not require a tracrRNA for function. Learn more about Cpf1 editing efficiency and TTTV site frequency in this article.

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One-step strategy to create transgenic and KO mouse models (Easi-CRISPR) uses Megamer™ ssDNA donors and CRISPR RNPs

Read how the use of long, ssDNA donor sequences can improve HDR editing efficiency. These donor sequences, now available as custom Megamer Single-Stranded DNA Fragments from IDT, are injected into mouse embryos, along with CRISPR RNP complexes, to create transgenic and conditional knockout mouse models.

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Using CRISPR genome editing for gene knockout and homology-directed repair (HDR)

Webinar review: Watch our webinar recording for expert guidance on a complete CRISPR genome editing workflow, including available tools and protocols. Also, see what we have learned about homology-directed repair and a new option for repair templates.

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3 valuable functions of a fluorescently labeled CRISPR-Cas9 tracrRNA

Product spotlight: Use a fluorescently labeled CRISPR-Cas9 tracrRNA to monitor transfection and simplify screening for editing events.

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A simple method to detect on-target editing or measure genome editing efficiency in CRISPR experiments

Product spotlight: Learn why the Alt-R® Genome Editing Detection Kit, a PCR-based, T7 endonuclease I (T7EI) assay, is the recommended method for CRISPR mutation detection.

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The key to successful electroporation in CRISPR genome editing experiments

Product spotlight: The most efficient CRISPR-based genome editing results from delivering CRISPR reagents to cells as ribonucleoprotein (RNP) complexes. Should your cells require electroporation, learn why we recommend using Alt-R® electroporation enhancers to increase genome editing efficiency.

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CRISPR-Cpf1 expands genome editing to new target sites

Webinar summary: Learn how the Alt-R® CRISPR-Cpf1 System can be an effective alternative to CRISPR-Cas9 genome editing. See how Cpf1 compares to Cas9 for editing efficiency, and what is the best method for delivering the RNA and protein components to your cells.

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Simple model for point mutation correction uses ssDNA repair oligo and CRISPR-Cas9 RNP

Citation summary: This publication demonstrates how CRISPR-Cas9 ribonucleoprotein (RNP) used for DNA cleavage, and a ssDNA oligonucleotide used for repair, will correct single base mutations without collateral mutagenesis in the surrounding sequence. Read the authors' explanation for why this CRISPR reagent delivery format is so successful.

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Genome editing in C. elegans using the Alt-R® CRISPR System

Scientists in the Dernburg lab have successfully executed CRISPR genome editing in C. elegans. Read this research profile to learn about their approach to editing, homology directed repair, and progeny screening. An abbreviated method and link to a full protocol are also provided.

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Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency

Looking for ways to increase the genome editing activity in your CRISPR experiments? This quick test suggests that spending a little extra time to anneal CRISPR RNAs will provide improvement.

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CRISPR genome editing: 5 considerations for target site selection

Read how your genome editing experiments can be improved with 5 quick tips for target selection and with reagents from the Alt-R® CRISPR-Cas9 System.

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Genome editing: How stable is my CRISPR RNA:Cas9 RNP complex?

You can safely complex CRISPR RNAs with Cas9 in advance of your experiments and store these RNPs for future use. Store CRISPR RNPs at 4°C for up to 2 weeks, or at –80°C long-term. RNP complexes stored in this way provide the same high level of genome editing as freshly complexed RNPs.

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Successful CRISPR genome editing in hard-to-transfect cells (i.e., Jurkat cells)

Use the conditions presented here for Clone E6-1 Jurkat cells as a starting point for optimization of CRISPR reagent delivery in cell types requiring electroporation.

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Webinar: Alt-R® CRISPR-Cas9 System ribonucleoprotein delivery optimization

Webinar: Genome editing using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) provides excellent editing efficiency, while reducing off-target editing and cell death. Watch this recorded presentation to find out how to easily generate and deliver CRISPR RNAs and Cas9 nuclease in an RNP format, using the optimized Alt-R® CRISPR-Cas9 System.

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Improve your genome editing with the Alt-R® S.p. Cas9 Nuclease 3NLS and modified crRNAs

Product Spotlight: Methods for CRISPR-Cas9 genome editing are diverse, but not all of them perform equally well. Learn about the Alt-R® S.p. Cas9 Nuclease 3NLS that, when used in combination the optimized Alt-R CRISPR crRNA and tracrRNA, provides a highly effective editing solution that is also easy to use.

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No Cas9 PAM NGG sequence in your target region for genome editing?

A restricted or AT-rich target region for genome editing experiments may lack a PAM NGG motif recognized by S. pyogenes Cas9. The Acidaminococcus sp. BV3LC Cpf1 enzyme uses a PAM site of TTTV (i.e., TTTA, TTTC, TTTG). It is therefore especially useful for targeting AT-rich regions. Read about this and other alternatives when targeting sequences with few or no NGG sites.

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RNA-guided gene drives for inheritance bias in yeast: Safe, responsible genome editing

Citation summary: Read about how a group of researchers use gBlocks® Gene Fragments and novel precautionary measures to responsibly investigate Cas9-based eukaryotic inheritance bias of gene drives.

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6 pieces of data that will change how you set up your CRISPR-Cas9 experiments

To improve the efficiency of CRISPR-Cas9 genome editing, IDT scientists evaluated several factors—Cas9 delivery, crRNA and tracrRNA length, which gRNA formats provide the most efficient on-target editing and elicit the least toxicity, the importance of protospacer size and site selection—that influence how we design and perform genome editing experiments. Review the data and results for 6 important factors that were addressed. These experimental findings resulted in a set of potent CRISPR tools that are now offered as the Alt-R® CRISPR-Cas9 System.

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CRISPR guide RNA format affects genome editing outcomes

Learn how the use of different formats for CRISPR guide RNAs can lead to different genome editing outcomes. The optimized, short RNA oligos that make up the crRNA and tracrRNA components of the Alt-R® CRISPR-Cas9 System outperform other CRISPR guide RNA formats. In addition to their improved editing efficiency, these short RNA oligos are unable to incorporate into the target genome, avoiding a common problem associated with DNA constructs, to provide cleaner editing results.

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Getting started with Alt-R® CRISPR-Cas9 genome editing

Webinar summary: Learn about the components of the Alt-R® CRISPR-Cas9 System for improved genome editing. Get information on designing Alt-R CRISPR crRNA oligos, and review the genome editing protocol from the user guide.

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Consistent, high performance genome editing

Product spotlight: Looking to improve the performance of your CRISPR-Cas9 genome editing application? The Alt-R™ CRISPR-Cas9 System offers potent on-target editing, easy implementation, and reduced cellular toxicity.

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IDT has improved the efficiency of CRISPR-Cas9 genome editing

Webinar summary: Learn how research conducted at IDT led to the development of a potent new set of CRISPR-Cas9 genome editing tools.

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CRISPR-Cas9 Genome Editing

With Alt-R CRISPR-Cas9 System get the core components for improved on-target editing performance, while experiencing lower toxicity, and no innate cellular response.

Increase your editing efficiency ≫


Related Content

Product page:

Alt-R CRISPR-Cas9 System

Webinar:

New RNA tools for optimized CRISPR/Cas9 genome editing

Learn how research conducted at IDT led to the development of a potent new set of CRISPR-Cas9 genome editing tools.

Poster:

Quantitative Measurement of CRISPR/Cas9 Gene Editing at the Level of Genomic DNA for sgRNA Site Selection Algorithm Development

User guide:

Alt-R CRISPR-Cas9 System User Guide