Mutagenesis
Support and Educational Content

Generate codon balanced libraries for mutagenesis with trimer modifications

Incorporating oligo codon trimers into oligo libraries results in balanced encoding of amino acids and eliminates unwanted stop codons. Such oligo libraries are useful for mutagenesis experiments to prepare proteins for screening for potential improvements in biological function.

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Need a library of related DNA or RNA oligo sequences?

Build variability into your oligo sequences by incorporating Mixed Bases. We offer mixes of multiple base types as well as nonstandard and modified bases.

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Site-directed mutagenesis—improvements to established methods

Site-directed mutagenesis techniques have relied primarily on PCR and standard cloning methods. Read about some of the common cloning methods used for mutagenesis and how double-stranded DNA fragments (gBlocks Gene Fragments) can save you both time and money.

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Mutagenesis using gBlocks® Gene Fragments

Citation summary: Learn how just 3 synthetic, high fidelity, double-stranded gBlocks Gene Fragments were used to mutate 18 different sites over the entire exon 7, 1039 bp sequence.

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Towards real-time imaging in single, living cells

Learn how this research team is developing oligonucleotide technologies for detection and quantification of specific RNA transcripts in live cells using molecular beacons and gBlocks Gene Fragments..

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Oligonucleotide quality requirements for mutagenesis protocols

Importance of oligo purity and how IDT tests its oligos to ensure they meet these standards.

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Methods for site-directed mutagenesis

Review these traditional PCR-based methods for creating a specific mutation in a known sequence, in vitro. Then read our follow-up article, Site-directed mutagenesis—Improvements to established methods (see the "Additional reading" sidebar) which describes how you can generate the same types of mutations, more quickly and efficiently, using custom, synthetic dsDNA fragments.

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Using site-directed mutagenesis to elucidate structure: function relationships

Read how this research group uses site-directed mutagenesis to identify critical small RNA binding regions and transcription factor regulation in E. coli.

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gBlocks® Gene Fragments

Double-stranded DNA up to 2,000 kb—for quick, hands-off template construction and CRISPR genome editing.

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Related Articles

Mutagenesis Application Guide

An overview of in vitro mutagenesis approaches, tools to simplify mutagenesis experiments, protocols, and an extensive troubleshooting section.

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Methods for Site-Directed Mutagenesis

An overview of the main techniques used for mutating specific gene construct regions.

Read more ≫

Mutagenesis Using gBlocks® Gene Fragments

Read how just 3 synthetic, high fidelity, double-stranded gBlocks™ Gene Fragments were used to mutate 18 different sites over the entire exon 7, a 1039 bp sequence.

Read more ≫

Oligonucleotide Quality Requirements for Mutagenesis Protocols

Importance of oligo purity and how IDT tests its oligos to ensure they meet these standards.

Read more ≫

Using Site-directed Mutagenesis to Elucidate Structure: Function Relationships

Scientists use mutagenesis to identify specific small, noncoding RNAs in bacteria.

Read more ≫