Next Generation Sequencing
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Generate consistent, reliable exome sequencing results

xGen® Exome Research Panel

Focused to provide uniform and specific coverage of 19,396 genes, the xGen® Exome Research Panel spans 39 Mb of the human genome. This concentrated design, paired with excellent reproducibility and deeper, more uniform coverage, enables the most comprehensive exome sequencing available and reliable identification of coding sequence variants.

With the xGen Exome Research Panel you can:

  • Obtain consistent results with individually synthesized and quality controlled target capture probes that are manufactured to GMP standards
  • Achieve deep and uniform coverage to minimize the need for further analysis
  • Effortlessly integrate with common sequencing platforms including Ion Torrent™ and Illumina sequencers
  • Generate results quickly with 4-hour hybridization
  • Expand targets to just the specific non-coding regions you need with easy addition of xGen Lockdown Probes

Learn more about the benefits of the xGen Exome Research Panel.

Additional reading: Successful target enrichment

Read examples of how IDT target enrichment probes and panels have already facilitated your colleagues’ research:

Delivering comprehensive genomic profiling for clinical cancer care—Scientists at Foundation Medicine, Inc. are leading a transformation in cancer care by helping clinicians to select appropriate treatment options for each patient, informed by a thorough understanding of the molecular changes specific to their disease. Read about the use of xGen® Lockdown® Probes in their flagship FoundationOne® Test.

Towards providing personalized medicine—considerations for reliable NGS data—Scientists at Geneseeq Technology, Inc. demonstrate how they improved their target capture methods to increase accuracy in clinical diagnostics by using optimized blocking oligos and stringent hybridization conditions.

A targeted sequencing approach to enable enhanced sensitivity in variant detection—Using 120 nt biotinylated DNA probes from IDT (xGen Lockdown Probes), researchers at The Genome Institute of Washington University (St Louis, MO, USA) describe their hybrid capture approach for increasing sequencing depth (deep digital data) from highly multiplexed samples at a fraction of the cost of whole genome sequencing.

Understanding how distal regulatory elements control gene expression—Based on Chromosomal Conformational Capture technology, Capture-C, a method developed by the Hughes laboratory (Oxford University, United Kingdom), allows researchers to isolate distal regulatory elements that interact with specific promoters in 3-dimensional space. By combining Capture-C with target capture using xGen Lockdown Probes, and next generation sequencing, the researchers can interrogate the regulatory landscapes of hundreds of genes in a single experiment.

Insertion site detection and targeted RNA capture using next generation sequencing—Scientists at Cofactor Genomics use in-solution hybridization (target enrichment) to focus on regions of interest for next generation sequencing.

Author: Ellen Prediger, PhD, is a senior scientific writer at IDT.

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xGen® Blocking Oligos

Adapter blocking oligos increase the number of on-target reads by preventing non-specific binding during hybridization.

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Related Articles

Target Enrichment Facilitates Focused Next Generation Sequencing

The rationale and benefits of enriching subsets of the genome (target enrichment by hybrid capture) prior to sequencing.

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Improving Uniform Coverage of Targeted Sequences for NGS

The challenges faced in obtaining uniform coverage of NGS data and how IDT xGen® Lockdown® Probes are uniquely positioned to facilitate uniform sequence coverage.

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Towards Providing Personalized Medicine—Considerations for Reliable NGS Data

Geneseeq Technology, Inc. demonstrates how they improved their target capture methods to increase accuracy in clinical diagnostics by using optimized blocking oligos and stringent hybridization conditions.

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Delivering Comprehensive Genomic Profiling for Clinical Cancer Care Using Targeted Sequencing

Read how scientists at Foundation Medicine, Inc use hybrid selection in their FoundationOne® Test to help clinicians select patient specific treatment options.

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Insertion Site Detection and Targeted RNA Capture Using Next Generation Sequencing

Scientists at Cofactor Genomics use in-solution hybridization to focus on regions of interest for next generation sequencing.

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