Next Generation Sequencing
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Get the most out of your NGS samples—expandable tumor and disease target capture panels

xGen® Target Capture Pan-Cancer and Inherited Disease Panels

For accurate and repeatable, targeted next generation sequencing of disease-causing mutations and 12 cancer types, begin your project with xGen® Inherited Diseases Panel or xGen® Pan-Cancer Panel.

Designed with experts from the Emory Genetics Laboratory and the Cancer Genome Atlas, IDT xGen target capture panels contain individually synthesized and quality controlled xGen Lockdown® Probes that target genes and other sequence regions relevant to particular diseases or cancers.

With xGen sequencing panels you can:

  • Return consistent results with high reproducibility (Figure 1)
  • Obtain deep uniform coverage to enable accurate variant calling (Figure 2)
  • Spike into Illumina or Nimblegen panels to enhance their sensitivity
  • Easily expand these and other panels with specific custom xGen® Lockdown® Probes you chose

Achieve a higher level of performance.  Learn more about xGen Target Capture Panels.

Figure 1. Consistent Performance and High Reproducibility With xGen® Pan-Cancer Panel. A comparison of target-by-target coverage between 2 sample replicates captured using the xGen® Pan-Cancer Panel shows excellent reproducibility, with an R2 value of 0.9013, demonstrating the consistent, high performance of the panel. The data shown are from 1M reads obtained by sequencing on the MiSeq® System (Illumina) using 2 x 150 paired-end reads.

Figure 2. Highly Uniform Coverage from xGen® Inherited Diseases Panel. Uniformity of coverage of the target regions is represented here as proportion of targets with >0.2 x mean coverage (blue), >0.5 x mean coverage (green) and >1.0 x mean coverage (grey). The data demonstrate that with a mean coverage depth of 100X, 99% of the targets captured using the xGen® Inherited Diseases Panel will have >20X depth of coverage. Error bars indicate standard deviation from the mean. 

Product focus: Target capture reagents

xGen® Lockdown® Probes—target capture probe pools for NGS

xGen Lockdown Probes are pools of individually synthesized, quality controlled, and normalized hybridization probes. Use them to generate custom capture panels for targeted sequencing to enhance the performance of existing panels. xGen Predesigned Gene Capture Probe Pools are available for any human RefSeq coding gene. Select from predesigned and custom probes that offer:

  • Sensitive detection of SNPs, indels, CNV, LOH, and translocations
  • GMP compliance for clinical and diagnostics research
  • Flexibility to augment existing panels or create completely custom panels
  • Quick delivery

Discover more about xGen Lockdown Probes.

xGen Lockdown Panels

xGen Lockdown Panels are preconfigured, validated, and stocked pools of xGen Lockdown Probes for targeted next generation sequencing of defined gene families:

  • xGen Exome Research Panel
  • xGen Acute Myeloid Leukemia Panel
  • xGen Pan-Cancer Panel
  • xGen Inherited Diseases Panel
  • xGen Human ID Research Panel
  • xGen Human mtDNA Research Panel

Discover more about xGen Lockdown Panels.

xGen Lockdown Reagents—hybridization and wash kit

xGen Lockdown Reagents have been optimized to deliver deep, even coverage of targets captured using xGen Lockdown Probes and Panels. Achieve uniform coverage with hybridization and wash buffers that are optimized for target enrichment using xGen Lockdown Probes and Panels. A short, 4-hour hybridization protocol generates results quickly.

Discover more about xGen Lockdown Reagents.

xGen Blocking Oligos

xGen Blocking Oligos prevent adapter cross hybridization and minimize off-target capture, increasing specificity during targeted sequencing. Universal Blocking Oligos—TS Mix are designed for use with Illumina barcoded adapters. This ready-to-use mix effectively blocks dual- or single-index adapters to significantly improve on-target performance.

  • xGen Standard Blocking Oligos
  • xGen Custom Blocking Oligos
  • xGen Blocking Oligos for diagnostics—contact for information
  • xGen Blocking Oligos for Ion Torrent adapters

Discover more about xGen Blocking Oligos.

Additional reading

Analyzing the exome—focus your NGS analysis with high-performance target capture—Webinar review: Targeted sequencing capture, using probe pools or panels, can increase read depth and the number of samples per run, while decreasing sequencing cost and simplifying data analysis. See how using individually synthesized, quality checked, DNA target capture probes (xGen® Lockdown® Probes) covering the human exome (xGen Exome Research Panel) performs across a variety of metrics; and compares to other available exome panels.

NGS target capture recommendations for FFPE samples—Webinar review: Learn how it is possible to create high quality target capture libraries from formalin-fixed, paraffin-embedded samples. Dr Kristina Giorda presents an FFPE sample workflow with a concise explanation of DNA quality analysis and how quality assessment can be used to guide the amount of DNA input for NGS library preparation.

NGS detection of low frequency genetic variants using novel, molecular sequencing adaptors—Webinar review: Watch this webinar recording to learn about unique molecular adaptors and a high-performance target capture method for NGS analysis of low frequency variants.

Targeting cancer pathways: Sensitive, comprehensive detection of genomic alterations using a custom NGS panel—Citation summary: Learn how researchers use xGen Lockdown Probes to screen cancer samples for key genes related to targeted cancer therapies.

Review other DECODED Online newsletter articles on NGS applications.

You can also browse our DECODED Online newsletter for additional application reviews, lab tips, and citation summaries to facilitate your research.

Author: Ellen Prediger, PhD, is a senior scientific writer at IDT.

© 2015, 2017 Integrated DNA Technologies. All rights reserved. Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners. For specific trademark and licensing information, see

xGen® Blocking Oligos

Adapter blocking oligos increase the number of on-target reads by preventing non-specific binding during hybridization.

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