Next Generation Sequencing
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Improve target capture using xGen® Lockdown® Probes and Reagents with an optimized protocol

Target enrichment allows researchers to direct sequencing efforts toward relevant genomic regions or transcripts of interest. Focusing on smaller regions improves detection of SNPs, indels, and other genomic variants, for more accurate analysis of insertion sites of mobile or viral DNA elements and highly variable regions. Reducing the size of the target pool also reduces cost, because several samples can then be multiplexed for simultaneous sequencing.

xGen Lockdown Reagents is a kit of optimized hybridization and wash buffers that allows researchers to maximize the capabilities of xGen Lockdown Probes. All buffers in the kit have been developed using Tm measurements that maximize binding of target regions, while improving the release of non-target sequences during washing steps. xGen Lockdown Reagents deliver deeper and more even coverage than the SeqCap® EZ Hybridization and Wash Kit (NimbleGen) previously recommended for use with xGen Lockdown Probes and Panels (Figure 1).

Figure 1. Improved performance of xGen® Lockdown® Probes and Panels using xGen Lockdown Reagents. Target enrichment was performed using a DNA library, created from human genomic DNA (Coriell) and enriched for a 57 Kb region of the human genome. Hybridization and wash steps were performed using xGen Lockdown Reagents (IDT) or the SeqCap® EZ Hybridization and Wash Kit (NimbleGen). The enriched library was sequenced on an Illumina MiSeq® system. The data show (A) greater depth of coverage and (B) better coverage uniformity with xGen Lockdown Reagents.

Retrieve the current protocol for use with xGen Lockdown Reagents, Hybridization capture of DNA libraries using xGen Lockdown Probes and Reagents, at www.idtdna.com/xGen.

Product focus: Target capture reagents from IDT

xGen® Lockdown® Probes

xGen Lockdown Probes are pools of individually synthesized, quality controlled, and normalized hybridization probes. Use them to generate custom capture panels for targeted sequencing to enhance the performance of existing panels. xGen Predesigned Gene Capture Probe Pools are available for any human RefSeq coding gene. Select from predesigned and custom probes that offer:

  • Sensitive detection of SNPs, indels, CNV, LOH, and translocations
  • GMP compliance for clinical and diagnostics research
  • Flexibility to augment existing panels or create completely custom panels
  • Quick delivery

Discover more about xGen Lockdown Probes.


xGen Lockdown Panels

xGen Lockdown Panels are preconfigured, validated, and stocked pools of xGen Lockdown Probes for targeted next generation sequencing of defined gene families:

  • xGen Exome Research Panel
  • xGen Acute Myeloid Leukemia Panel
  • xGen Pan-Cancer Panel
  • xGen Inherited Diseases Panel
  • xGen Human ID Research Panel
  • xGen Human mtDNA Research Panel

Discover more about xGen Lockdown Panels.


xGen Lockdown Reagents Hybridization and Wash Kit

xGen Lockdown Reagents have been optimized to deliver deep, even coverage of targets captured using xGen Lockdown Probes and Panels. Achieve uniform coverage with hybridization and wash buffers that are optimized for target enrichment using xGen Lockdown Probes and Panels. A short, 4-hour hybridization protocol generates results quickly.

Discover more about xGen Lockdown Reagents.


xGen Blocking Oligos

xGen Blocking Oligos prevent adapter cross hybridization and minimize off-target capture, increasing specificity during targeted sequencing. Universal Blocking Oligos—TS Mix are designed for use with Illumina barcoded adapters. This ready-to-use mix effectively blocks dual- or single-index adapters to significantly improve on-target performance.

Also available are:

  • xGen Standard Blocking Oligos
  • xGen Custom Blocking Oligos
  • xGen Blocking Oligos for diagnostics—contact GMPinfo@idtdna.com for information
  • xGen Blocking Oligos for Ion Torrent adapters

Discover more about xGen Blocking Oligos.

Additional reading

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xGen® Blocking Oligos

Adapter blocking oligos increase the number of on-target reads by preventing non-specific binding during hybridization.

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