Alternative dyes for FISH, FRET, and qPCR

ATTO-labeled oligonucleotides offer additional alternatives for applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.* ATTO dyes are a group of patented fluorescent markers that feature strong absorption, photostability, and fluorescence quantum yield.

The ATTO dyes are perfect substitutes for dyes such as ROX™, VIC^®, LIZ^®, and Alexa Fluor^® dyes. For qPCR applications, dye calibration will be needed for each qPCR instrument [1].

Ordering ATTO dyes

Catalog ATTO dyes can be attached to 100 nmol, 250 nmol, and 1 μmol DNA and RNA oligonucleotides, or duplex products. To order, visit the DNA Oligos or RNA Oligos ordering pages, where you can choose an ATTO dye as either a 5′ or 3′ modification. All ATTO-labeled oligos from IDT will be HPLC-purified.

For questions about ATTO dye modifications or products not found on this list, including internal dye placement or dual-labeled probes, reach out at contact us.

Figure 1. Strong fluorescence of ATTO dyes. Rat stomach stained with mouse anti-smooth muscle α-actin antibody followed by ATTO 488 anti-mouse IgG (green); and polyclonal rabbit anti-cytokeratin followed by ATTO 647N anti-rabbit IgG (red) [Image courtesy of ATTO-TEC GmbH]. For detailed information on these (and other) ATTO dyes, visit ATTO-TEC.

*ATTO dyes have not been tested on Illumina^® platforms for use with certain fluorescent applications, such as sequencing, genotyping, and fragment analysis. Researchers must perform independent testing for such use.