PCR and qPCR
Support and Educational Content

qPCR assay plates for large and high throughput studies

PrimeTime® qPCR Assay Plates and PrimeTime qPCR Primer Plates

PrimeTime® qPCR Assays for 5’ nuclease assays and PrimeTime qPCR Primers for use with intercalating dye (e.g., SYBR® Green) assays are now available in 96-well plates. Enjoy all the benefits of IDT PrimeTime products, including accurate assay design using current information from NCBI, gold standard performance, and compliance with MIQE Guidelines.

  • Fully customizable—Use the state-of-the-art custom plate design tool for ordering. Design your own master plate; just cut, copy, paste, and fill in as you would with Microsoft Excel.
  • Versatile—Choose from predesigned assays for human, mouse, or rat; or simply enter your own primer and probe sequences manually.
  • Convenient—Get assays with different dye–quencher combinations in the same plate.
  • Affordable—No need to purchase a full 96-well plate. All that is required is a minimum order of 24 assays or primer pairs per plate.
  • Value—Generate your own replicate plates for lower cost per reaction.

For more information, or to order PrimeTime qPCR Plates, go to www.idtdna.com/primetime or email us at custcare@idtdna.com.

Product focus: qPCR Reagents—everything but your sample

All the reagents you need for successful qPCR assays are available through IDT.

Related reading

Strategies for optimizing high throughput qPCR for expression profiling—Webinar summary: Learn how to address the challenges of high throughput RT-qPCR expression profiling from a prominent qPCR expert, Dr. Mikael Kubista (TATAA Biocenter, Sweden).

qPCR Probes—selecting the best reporter dye and quencher—Read these recommendations for choosing dyes and quenchers, taking into account instrument compatibility and multiplex probe applications.

Do your qPCR assays come with sequence information? They should. Here Is why—qPCR assays (primer & probe sets) from other suppliers are often provided without sequence information. IDT always gives you the sequences to the oligos you order. And that can be very important. Read why.

Considering SNPs when designing PCR and qPCR assays—NGS has led to a dramatic increase in identified SNPs. SNPs can pose a problem when they underlie primer or probe sequences used in PCR/qPCR. Learn what effect they can have and how you can minimize their impact on your PCR assays.

Review other DECODED Online newsletter articles on PCR and qPCR applications.

You can also browse our DECODED Online newsletter for additional application reviews, lab tips, and citation summaries to facilitate your research.

Author: Nicola Brookman-Amissah, PhD, is a senior scientific writer at IDT.

© 2014, 2017 Integrated DNA Technologies. All rights reserved. Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners. For specific trademark and licensing information, see www.idtdna.com/trademarks.


Predesigned qPCR Assays

Probe-based qPCR assays for quantification of human, mouse, and rat gene expression. Order in plates or tubes.

Search human, mouse, or rat genes ≫

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