PCR and qPCR
Support and Educational Content

qPCR probes—get the right scale at the right price

PrimeTime® Eco qPCR Probes provide a mid-range scale at a lower price per reaction

IDT now offers FAM-labeled, double-quenched qPCR probes at a mid-range scale for researchers who need 100–500 amplification reactions, without having to pay for a much larger amount of probe. The PrimeTime® Eco qPCR Probe is provided at 2.5 nmoles normalized yield, and is enough for approximately 500 amplifi­cation reactions. It is available only with a FAM/ZEN™/IBFQ® dye/ quencher combination. This double-quenched probe includes an internal quencher, the ZEN quencher, which provides greater background reduction and increased signal detection. For an example of how ZEN Double-Quenched Probes can improve real-time PCR data, see Figure 1, in the article, Two Quenchers are Better Than One!

* IBFQ = Iowa Black Fluorescent Quencher

Predesigned qPCR Assays

Probe-based qPCR assays for quantification of human, mouse, and rat gene expression. Order in plates or tubes.

Search human, mouse, or rat genes ≫

Related Articles

Designing PCR Primers and Probes

General guidelines for designing primers and probes and for choosing target locations for PCR amplification.

Read more ≫

Steps for a Successful qPCR Experiment

Considerations for 5′ nuclease assay design and experimental setup to help you obtain accurate and consistent results.

Read more ≫

Interpreting Melt Curves: An Indicator, Not a Diagnosis

Examining PCR melt curve data to determine what it can/cannot tell us about resulting PCR amplicons.

Read more ≫

Epigenetic Biomarkers for Prostate Cancer

Scientists use methylation and expression analysis methods to evaluate epigenetic markers for early, noninvasive detection of aggressive prostate cancer. IDT PrimeTime® qPCR Assays, ZEN™ Double-Quenched Probes, and gBlocks® Gene Fragments facilitate this research.

Read more ≫

Optimizing Multiplex qPCR for Detecting Infectious Diseases and Biothreat Agents in the Field—ZEN™ Double-Quenched Probes bring down the background

Tetracore researchers developing large sets of robust probe-based qPCR assays discuss the need to: use probe dyes compatible on common PCR instruments, maintain low background with multiple probes, and reformulate assays to address viral mutation.

Read more ≫