PCR and qPCR
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MGB Eclipse® Probes for human in vitro diagnostics

How MGB qPCR probes are used

As a result of improved knowledge and understanding of the human genome, quantitative real-time PCR (qPCR) has become an increasingly important tool for in vitro diagnostics and patient management. The gold standard, 5′ nuclease qPCR assay uses oligonucleotide probes that have 3 critical features:

  • fluorescent reporter dyes
  • quenchers for these dyes
  • a minor groove binding groups

The technique is routinely used for various clinical tests, including identification of mutations that are related to cancer development or metastasis, gene defects that result in genetic diseases (e.g., cystic fibrosis), prenatal testing to detect congenital disorders, and detection of viral and bacterial DNA in human samples.

MGB probe

MGB qPCR probes with IDT quality

MGB Eclipse® Probes, a widely used type of qPCR probe, are comprised of oligonucleotides coupled to fluorescent dyes, Eclipse Dark Quenchers, and Minor Groove Binder (MGB™) groups. You can now get MGB Eclipse Probes made by IDT, which provide the oligonucleotide quality and GMP manufacturing expertise our company is known for (review comparison data). MGB Eclipse Probes are licensed by ELITech for use solely in human in vitro diagnostic end-use applications.

How to order and what you get

To help diagnostic labs keep up with the demand, we offer easy ordering through our website. Simply upload a spreadsheet, then choose your fluorophore and normalized final yield amount. If your lab needs HPLC purification, you can easily add that service as well. Standard desalt products typically ship in 2 weeks, purified products ship in 3 weeks. Diagnostic test providers can also be assured of complete transparency and product traceability, which are hallmarks of our GMP business strategy.

Learn more or order now.

For use as a component in the Purchaser's Human IVD applications only.


Author: Maureen Young, PhD, is a senior scientific writer at IDT.


Product focus—GMP products and services

IDT is a contract GMP manufacturer of oligonucleotide reagent components for in vitro diagnostic devices (IVDs) and analyte-specific reagents (ASRs) for laboratory-developed tests (LDTs). Our GMP Manufacturing services include dedicated ordering, production, final-fill, and shipping systems that meet the elevated quality standards required for products used in clinical or molecular diagnostic applications. IDT GMP facilities are ISO 13485:2003 certified, compliant with US Food & Drug Administration (FDA) Quality System Regulation (QSR) 21 CFR Part 820, and US FDA registered.

From research to Dx commercialization, IDT can help you get there.

Learn more or request a quote at http://www.idtdna.com/pages/products/gmp-manufacturing/.

Additional reading

Time to go GMP?—Learn how GMP manufacture of oligos for diagnostic use can provide your organization with defined specifications and control over manufacturing processes.

Intact, single cell analysis of protein and nucleic acid biomarkers for clinical diagnostics—One of IDT’s GMP customers, IncellDx is a unique molecular diagnostics company with high throughput cellular analysis technologies and assays that can simultaneously identify specific protein, RNA, and DNA species within intact cells. Read about their technology and the product quality and services the IDT GMP division provides.

Oligos for molecular diagnostics—Understand the difference between Good Manufacturing Practices (GMP) and ISO 13485 certification, and what these credentials mean for oligonucleotides manufactured for human diagnostics.

Fluorescent dyes with no licensing restrictions—a growing portfolio—Need fluorescent dyes suitable for commercial and diagnostic applications? These have no patent licensing restrictions. Review this table of Freedom Dye alternatives for commonly used proprietary dyes.


© 2015, 2016 Integrated DNA Technologies. All rights reserved. Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners. MGB is a trademark and MGB Eclipse is a registered trademark of Elitech Group. For specific trademark and licensing information, see www.idtdna.com/trademarks


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