Ideas to Streamline Your Research
Housekeeping genes commonly used as internal controls, often may not be the best choices due to lesser known functions and the presence of pseudogenes, some of which, in the case of GAPDH---used here as an example--may be expressed.
An easy way to combine control templates/multiple targets onto a single construct and the advantages that can provide for PCR experiments.
Recommendations for oligonucleotide purification based on oligo length, application, yield required, and presence of modifications.
Explanations for why sequenced clones sometimes show deletions and some suggestions for mitigating this result.