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Product Spotlight
Your Favorite Products and Their Applications

Generate codon balanced libraries for mutagenesis with trimer modifications

Incorporating oligo codon trimers into oligo libraries results in balanced encoding of amino acids and eliminates unwanted stop codons. Such oligo libraries are useful for mutagenesis experiments to prepare proteins for screening for potential improvements in biological function.

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N6-methyladenosine (m6A)—modulating RNA localization, structure, stability, splicing, and translation

Modification Highlight: Read about the mechanism of methylation and functions of N6-methyladenosine (m6A) modifications in the cell. N6-methyladenosine is available as a popular non-catalog modification from IDT, providing a substrate for studying the role of this natural, reversible, post-translational modification.

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Valuable support for genomics research

Product Spotlight: Take advantage of the full list of products, services, and support IDT provides for genomics research. Whether you are performing sequencing, gene expression, genome editing, or synthetic biology experiments, IDT has cost-effective, rapid, high quality, and high throughput solutions.

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Insert an abasic site into your sequence

Use the dSpacer, rSpacer, and Abasic II modifications to introduce abasic sites into DNA or RNA oligonucleotides. These modifications create a single base space that replicates the loss of base pairing ability by a nucleotide.

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Need a library of related DNA or RNA oligo sequences?

Build variability into your oligo sequences by incorporating Mixed Bases. We offer mixes of multiple base types as well as nonstandard and modified bases.

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Increase the Tm of short, AT-rich primers and probes

Modification highlight: Add this modified base to increase the melting temperature (Tm) of primers and probes. It is especially useful when you need to work with short A-T rich sequences.

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Inverted bases

Learn how inverted bases allow you to reverse the orientation of part of your oligo sequence or add a 5-end restricted modification to the 3 end of your sequence.

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Improved target capture using xGen® Lockdown® Probes and Reagents with an optimized protocol

xGen Lockdown Reagents and new hybridization capture protocol for xGen Lockdown Probes and Panels reveal the increased potential of IDT target enrichment products.

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Getting started with Alt-R™ CRISPR-Cas9 genome editing

Webinar: Watch a recording of our webinar to learn about the components of the Alt-R CRISPR-Cas9 System, get information on designing Alt-R CRISPR crRNA oligos, and review the genome editing protocol from the user guide.

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Consistent, high performance genome editing

Product spotlight: Looking to improve the performance of your CRISPR-Cas9 genome editing application? The Alt-R™ CRISPR-Cas9 System offers potent on-target editing, easy implementation, and reduced cellular toxicity.

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Obtain high efficiency qPCR results using PrimeTime® Gene Expression Master Mix

Product Spotlight: If you are doing 5′ nuclease assays for qPCR or 2-step qPCR, we have a reliable master mix for you to use in singleplex or multiplex.

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Super G® (8-aza-7-deazaguanosine) bases

Modification Highlight: Super G bases are analogs of guanine that eliminate naturally occurring, non-Watson-and-Crick secondary structures associated with guanine-rich sequences. Substitution of a single guanine in a run of G residues with a Super G modified base can increase oligo yield and purity, eliminate secondary structure, improve probe-based qPCR signal, and increase duplex stability and mismatch discrimination.

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Increase sensitivity and precision in your qPCR experiments

Learn how you can use double-quenched probes to decrease background, and increase sensitivity and precision in your qPCR experiments.

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gBlocks® Gene Fragments in Plates

Obtain gBlocks Gene Fragments in 96-well plates to facilitate large orders and high throughput use.

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Get the most out of your NGS samples

Expandable NGS target capture panels that enrich for mutated genes implicated in tumors and genes associated with inherited diseases. Both panels return consistent results with high reproducibility and deep uniform coverage.

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Generate consistent, reliable exome sequencing results

Spanning 39 Mb of the human genome, the xGen® Exome Research Panel was designed to provide uniform and specific coverage of the coding regions for 19,396 genes. Easily and cost-effectively expand this panel to include specific non-coding target regions through addition of xGen® Lockdown® Probes.

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MGB Eclipse® Probes for human in vitro diagnostics

Product spotlight: Obtain MGB Eclipse® Probes for human in vitro diagnostic end-use applications. Selecting MGB Eclipse® Probes made by the IDT GMP manufacturing division provides you with complete process transparency and product traceability, in addition to consistent, reliable oligonucleotide quality.

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NGS Target Capture—Achieve Deeper Coverage and Better Uniformity

Preconfigured pools of probes each targeting an individual coding sequence (CDS) for all human protein-coding genes.

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gBlocks® Gene Fragments Ordering Tool

Easy tool for uploading or entering gBlocks Gene Fragments sequence requests, that also judges complexity, and allows you to edit the sequences on the spot.

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Longer gBlocks® Gene Fragments Make Gene Assembly Simple

Made-to-order, double-stranded DNA fragments up to 2 kb simplify cloning and mutagenesis protocols.

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RxnReady Oligos—get your oligos premixed!

Have 2–6 standard desalted DNA oligonucleotides premixed in a single tube according to your specifications. This can be useful when performing multiplex PCR, or when generating sets of insertions or deletions through site-directed mutagenesis.

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Fluorescent dyes with no licensing restrictions—a growing portfolio

Fluorescent dyes suitable for commercial and diagnostic applications and that have no patent licensing restrictions. A table of Freedom Dye alternatives for commonly used proprietary dyes is provided.

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Targeted sequencing for acute myeloid leukemia

An NGS target enrichment panel composed of >11,500 xGen Lockdown® Probes targeting 260 genes implicated in acute myeloid leukemia (AML).

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Accelerate Your Recombinant Gene or Protein Development

Library of variant dsDNA sequences to generate up to 4E18 sequence variations that could be used for a variety of functional screenings of nucleotide or peptide variants.

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Libraries of double-stranded DNA fragments

Learn about obtaining double-stranded DNA fragment libraries that contain up to 18 consecutive N or K bases for generating up to 4e18 sequence variations.

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Beginning genotyping experiments?

Product spotlight: When starting genotyping experiments, reduce waste and spend less by ordering the small size of LNA Probes—enough for 100 rxns—ideal for preliminary experiments.

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qPCR Probes—Get the Right Scale at the Right Price

FAM-labeled, double-quenched qPCR probes at a mid-range scale for researchers who only need 100–500 amplification reactions – cheaper than larger reaction size.

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qPCR Assay Plates for Large and High Throughput Studies

Read about your ability to customize qPCR assay plates for probe-based or primer alone assays. You can mix different dye-quencher combinations and request replicate plates. And no need to fill every well.

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Blocking oligos for NGS target enrichment applications

Product Spotlight: Learn how blocking oligos,added during the hybridization step of NGS target capture reactions, bind to the adapter sequences to prevent them from hybridizing to each other. Preventing this non-specific binding results in an increased number of on-target reads.

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Alternative dyes for FISH, FRET, and qPCR

Use ATTO™ dye labeled oligonucleotides as alternatives in applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.

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Target Capture Probes for Next Generation Sequencing

Individually synthesized, biotinylated probes used to augment target capture of array-derived probes in NGS applications and to achieve deeper, more uniform coverage.

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Making RNAi Research Easy

IDT can supply any of the forms of interfering RNA you need.

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Cloning—Ultramer® Oligos vs. Standard Oligos

Ultramer oligonucleotides make it easy to add/delete sequence elements, anneal oligos to create dsDNA inserts,and to include degenerate bases to mimic SNP.

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Need Small Amounts of Many Oligos?

DNA Plate Oligos are now available in 500 pmole amounts.

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Reduce Barcode Misalignment During Sequencing

The TruGrade Processing Service for oligonucleotides reduces NGS barcode misalignment.

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qPCR with intercalating dyes: PrimeTime® qPCR Primers

PrimeTime qPCR Primers are now available to detect genes in human, mouse, and rat transcriptomes with intercalating dyes such as SYBR® Green and EvaGreen®

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Inhibiting miRNAs using Antisense Oligonucleotides

Learn about Anti-miRNAs for knockdown of miRNA expression in loss/gain of function studies.

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5-Hydroxymethyl-dC

Naturally occurring 5-hydroxymethylcytosine (5-hmC) has been hypothesized to be an intermediate in a demethylation pathway or as an additional epigenetic factor. Learn more about this exciting new modification.

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Assembling Gene Fragments using Isothermal Assembly

Use of the Gibson isothermal assembly method to combine gene fragments such as IDT gBlocks Gene Fragments.

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Easy Gene Assembly: gBlocks® Gene Fragments

gBlocks Gene Fragments are synthetic double-stranded DNA that can be used for almost any cloning application, available from 125—500 bp.

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200 pmol Ultramer® DNA Plate Oligo

Ultramer high-fidelity, long oligonucleotides now available in 200 pmol plates.

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More ZEN/Fluorophore Combos!

Including a second quencher in one's qPCR probe will increase assay sensitivity and provide improved precision.

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qPCR probes—high Tms without sacrificing quenching

Placed directly between DNA bases, the ZEN Internal Quencher eliminates the need for LNAs, MGB, or internal quenchers attached to thymidine bases. When combined with a 3’ quencher, you can design longer probes with sufficient melting temperature for qPCR while maintaining a consistently low background.

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Up-to-date assay design

Keeping up to date with the continually increasing number of annotated SNPs is critical when designing qPCR assays, particularly when working with samples that may have different or unknown genetic backgrounds.

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Click chemistry-generated, internal dye-labeled oligonucleotides

Learn about "click chemistry" reactions, used to join small chemical subunits in a modular fashion, yielding singular reaction products that are typically physiologically stable and stereospecific.

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Using qPCR assays in multiplex experiments

Product spotlight: IDT PrimeTime qPCR Assays offer multiple dye/quencher combinations and primer/probe ratios to simplify the multiplex experiment design.

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Mutagenesis Made Easy with Ultramer Oligos

Ultramer primers are oligos that range in length from 25–200 bases. These long primers are especially useful in PCR and site-directed mutagenesis reactions.

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Ultramer Oligonucleotides

Ultramers and miniGenes as positive controls

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SYBR® to 5’ nuclease assays

Product spotlight: Researchers often use intercalating dyes, like SYBR® Green I, for qPCR experiments for the advantage they bring in lowered price and reduced turnaround time. However, the disadvantages to using these reagents are significant. Read how easy it is to convert to 5’ nuclease assays and the advantages the addition of a hydrolysis probe will provide.

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LNA Calculator Updates in OligoAnalyzer

The free, online OligoAnalyzer tool is used to analyze the physical properties of oligonucleotide sequences.

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PrimeTime® Predesigned qPCR Assays

PrimeTime Predesigned qPCR Assays are now available for human, mouse, and rat transcriptomes in the NCBI database.

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