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Product Spotlight
Your Favorite Products and Their Applications

Modification Highlight: Super G (8-aza-7-deazaguanosine)

Super G is an analog of guanine that eliminates naturally occurring, non-Watson-and-Crick secondary structures associated with guanine-rich sequences. Substitution of a single guanine in a run of G residues with a Super G modified base can increase oligo yield and purity, eliminate secondary structure, improve probe-based qPCR signal, and increase duplex stability and mismatch discrimination.

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Increase sensitivity and precision in your qPCR experiments

Learn how you can use double-quenched probes to decrease background, and increase sensitivity and precision in your qPCR experiments.

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gBlocks® Gene Fragments in Plates

Obtain gBlocks Gene Fragments in 96-well plates to facilitate large orders and high throughput use.

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Get the most out of your NGS samples

Expandable NGS target capture panels that enrich for mutated genes implicated in tumors and genes associated with inherited diseases. Both panels return consistent results with high reproducibility and deep uniform coverage.

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Generate consistent, reliable exome sequencing results

Spanning 39 Mb of the human genome, the xGen® Exome Research Panel was designed to provide uniform and specific coverage of the coding regions for 19,396 genes. Easily and cost-effectively expand this panel to include specific non-coding target regions through addition of xGen® Lockdown® Probes.

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Product Spotlight: MGB Eclipse Probes for human in vitro diagnostics

Obtain MGB Eclipse® Probes for human in vitro diagnostic end-use applications. Selecting MGB Eclipse® Probes made by the IDT GMP manufacturing division provides you with complete process transparency and product traceability, in addition to consistent, reliable oligonucleotide quality.

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NGS Target Capture—Achieve Deeper Coverage and Better Uniformity

Preconfigured pools of probes each targeting an individual coding sequence (CDS) for all human protein-coding genes.

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Target Enrichment Protocol for Next Generation Sequencing

A protocol for Rapid Capture (4 hr hybridization) is posted on xGen Lockdown webpage (www.idtdna.com/xgen.)

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gBlocks® Gene Fragments Ordering Tool

Easy tool for uploading or entering gBlocks Gene Fragments sequence requests, that also judges complexity, and allows you to edit the sequences on the spot.

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Longer gBlocks® Gene Fragments Make Gene Assembly Simple

Made-to-order, double-stranded DNA fragments up to 2 kb simplify cloning and mutagenesis protocols.

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Fluorescent Dyes With No Licensing Restrictions—A Growing Portfolio

Fluorescent dyes suitable for commercial and diagnostic applications and that have no patent licensing restrictions. A table of Freedom Dye alternatives for commonly used proprietary dyes is provided.

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Targeted Sequencing for Acute Myeloid Leukemia

An NGS target enrichment panel composed of >11,500 xGen Lockdown® Probes targeting 260 genes implicated in acute myeloid leukemia (AML).

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Accelerate Your Recombinant Gene or Protein Development

Library of variant dsDNA sequences to generate up to 4E18 sequence variations that could be used for a variety of functional screenings of nucleotide or peptide variants.

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Beginning Genotyping Experiments?

Small size of LNA Probes—enough for 100 rxns—ideal for preliminary experiments. Less cost and waste.

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qPCR Probes—Get the Right Scale at the Right Price

FAM-labeled, double-quenched qPCR probes at a mid-range scale for researchers who only need 100–500 amplification reactions – cheaper than larger reaction size.

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qPCR Assay Plates for Large and High Throughput Studies

Read about your ability to customize qPCR assay plates for probe-based or primer alone assays. You can mix different dye-quencher combinations and request replicate plates. And no need to fill every well.

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Superior Primer Design Software: The PrimerQuest® Tool

The updated PrimerQuest tool enables even better qPCR designs by linking directly to NCBI databases, allowing the use of multiple formats of gene identification annotations.

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Target Capture Probes for Next Generation Sequencing

Individually synthesized, biotinylated probes used to augment target capture of array-derived probes in NGS applications and to achieve deeper, more uniform coverage.

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Making RNAi Research Easy

IDT can supply any of the forms of interfering RNA you need.

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Cloning—Ultramer® Oligos vs. Standard Oligos

Ultramer oligonucleotides make it easy to add/delete sequence elements, anneal oligos to create dsDNA inserts,and to include degenerate bases to mimic SNP.

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Need Small Amounts of Many Oligos?

DNA Plate Oligos are now available in 500 pmole amounts.

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Reduce Barcode Misalignment During Sequencing

The TruGrade Processing Service for oligonucleotides reduces NGS barcode misalignment.

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qPCR with Intercalating Dyes: PrimeTime® qPCR Primers

PrimeTime qPCR Primers are now available to detect genes in human, mouse, and rat transcriptomes with intercalating dyes such as SYBR® Green and EvaGreen®

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Inhibiting miRNAs using Antisense Oligonucleotides

Learn about Anti-miRNAs for knockdown of miRNA expression in loss/gain of function studies.

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5-Hydroxymethyl-dC

Naturally occurring 5-hydroxymethylcytosine (5-hmC) has been hypothesized to be an intermediate in a demethylation pathway or as an additional epigenetic factor. Learn more about this exciting new modification.

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Assembling Gene Fragments using Isothermal Assembly

Use of the Gibson isothermal assembly method to combine gene fragments such as IDT gBlocks Gene Fragments.

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Easy Gene Assembly: gBlocks® Gene Fragments

gBlocks Gene Fragments are synthetic double-stranded DNA that can be used for almost any cloning application, available from 125—500 bp.

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200 pmol Ultramer® DNA Plate Oligo

Ultramer high-fidelity, long oligonucleotides now available in 200 pmol plates.

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More ZEN/Fluorophore Combos!

Including a second quencher in one's qPCR probe will increase assay sensitivity and provide improved precision.

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qPCR probes: high Tms without sacrificing quenching

Placed directly between DNA bases, the ZEN Internal Quencher eliminates the need for LNAs, MGB, or internal quenchers attached to thymidine bases. When combined with a 3’ quencher, you can design longer probes with sufficient melting temperature for qPCR while maintaining a consistently low background.

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Up-to-Date Assay Design—Avoiding SNPs

Keeping up to date with the continually increasing number of annotated SNPs is critical when designing qPCR assays, particularly when working with samples that may have different or unknown genetic backgrounds.

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Click Chemistry-Generated Internal Dye-Labeled Oligonucleotides

In general terms, “Click Chemistry” describes reactions used to join small chemical subunits in a modular fashion, yielding singular reaction products that are typically physiologically stable and stereospecific.

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Have Your Oligos Premixed

Researchers often mix oligonucleotides to create multiple primers for multiplex PCR or to generate sets of insertions or deletions through site-directed mutagenesis. IDT will now make those mixes for you, reducing bench time and errors due to contamination, so that you can spend more time on discovery and less time pipetting.

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Using PrimeTime qPCR Assays in Multiplex Experiments

IDT PrimeTime qPCR Assays offer multiple dye/quencher combinations and primer/probe ratios to simplify the multiplex experiment design.

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Mutagenesis Made Easy with Ultramer Oligos

Ultramer primers are oligos that range in length from 25–200 bases. These long primers are especially useful in PCR and site-directed mutagenesis reactions.

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Ultramer Oligonucleotides

Ultramers and miniGenes as positive controls

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PrimeTime qPCR Assays

Demand the most from your qPCR experiments! Flexible, Affordable, Gold-standard Performance.

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LNA Calculator Updates in OligoAnalyzer

The free, online OligoAnalyzer tool is used to analyze the physical properties of oligonucleotide sequences.

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PrimeTime® Predesigned qPCR Assays

PrimeTime Predesigned qPCR Assays are now available for human, mouse, and rat transcriptomes in the NCBI database.

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