qPCR with Intercalating Dyes: PrimeTime® qPCR Primers

PrimeTime® qPCR Primers are primer sets predesigned to detect genes in human, mouse, and rat transcriptomes. These primer sets are ideal for use with intercalating dyes such as SYBR® Green and EvaGreen®, where no probe is needed, and they eliminate the inconvenience of designing specific assays for intercalating dyes. Now you can simplify the transition from discovery to screening by using PrimeTime qPCR Primers to analyze large sample numbers in initial discovery before upgrading to probe-based assays (PrimeTime qPCR Assays) for improved specificity and performance as sample numbers decrease.

The benefits of PrimeTime qPCR Primers include:

  • Up-to-date sequence design—While primer binding sites are carefully pre-chosen using a sophisticated design engine, primers are only synthesized at the time of order using current sequence information to avoid SNPs. A BLAST search is also performed to eliminate off-target effects.
  • Guaranteed performance, high efficiency—Average qPCR efficiencies are >90% and gel analysis consistently shows single bands for PCR products.
  • Compatible with popular master mixes—Comparable efficiencies are achieved when the primers are used with various commercial master mixes under manufacturers’ cycling conditions. See the data under PrimeTime Product Performance.
  • Easy ordering and sequence provided—Simple assay selection interface with sequence information provided upon order placement.

To order, use the PrimeTime qPCR Assay Selection Tool or the RealTime PCR Tool. You can also use the RealTime PCR tool to design and order custom primers (Note: the performance of custom designed primers is not guaranteed by IDT). PrimeTime qPCR Primers ship in 2–3 business days.