Small RNAs/Functional Genomics
Support and Educational Content

Guaranteed knockdown of RNA in loss-of-function studies

Product spotlight: Predesigned DsiRNAs in the TriFECTa Kits are guaranteed to knockdown gene expression in RNA interference (RNAi) studies.

Read More>


Enzyme-linked DNA aptamer assay provides advantages over standard immunoassays

Citation summary: Find out how this research team used candidate aptamers in an enzyme-linked aptamer sorbent assay (ELASA) to detect human insulin-like growth factor-I, a biomarker for recombinant human growth hormone, used in athletic doping.

Read More>


Planning to work with aptamers?

We are often asked whether IDT manufactures aptamers. The answer is, yes! IDT does synthesize aptamers and aptamer libraries, and there are already 100s of published research papers describing the successful use of such sequences manufactured by IDT. Learn about aptamers, SELEX, and how IDT can assist you with reagents for your aptamer applications.

Read More>


Antisense Oligonucleotides (ASOs)

Modification Highlight: Antisense oligos are the first oligonucleotide-based approach for disrupting gene expression. Learn about new applications for antisense oligos, including the study of lncRNA function.

Read More>


Tips for successful lncRNA knockdown: Design, delivery, and analysis of antisense and RNAi reagents

IDT research scientist Kim Lennox has been optimizing effective lncRNA knockdown with antisense and RNAi reagents. Here she provides some tips for successful lncRNA knockdown.

Read More>


A new renaissance for antisense in the era of lncRNA

Noncoding RNAs such as lncRNA, are much more prevalent in humans than protein-coding RNA. Antisense oligonucleotides (ASO), previously used for knockout experiments, are being employed to study the role of noncoding RNAs in gene regulation. ASOs provide several advantages over siRNAs (and DsiRNA) for this purpose.

Read More>


Nano-delivery of DsiRNAs results in improved gene silencing and anticancer activity

Citation summary: Find out how a dendrimer-based, targeted nano-delivery system that uses Dicer Substrate RNAs (DsiRNAs) leads to improved gene silencing and anticancer activity in prostate cancer models in vitro and in vivo.

Read More>


Nanoparticle encapsulation method delivers DsiRNA to difficult-to-transfect cells

Research profile: Precision NanoSystems Inc has developed unique reagents and equipment that enable the simple assembly of novel nanoparticles, resulting in greatly improved delivery of RNA payloads. Learn about use of products to deliver DsiRNAs, 27-mer siRNAs, into primary hippocampal neuron cells and neuronal cells in vivo.

Read More>


Using DsiRNA to map pain pathways in the CNS

DsiRNAs (Dicer-substrate RNAs) are chemically synthesized 27mer duplex RNAs that have increased potency in RNA interference compared to traditional 21mer siRNAs. Read this example of a research group using the technology for in vivo delivery to the CNS.

Read More>


Advantageous properties of Dicer-Substrate Interfering RNA (DsiRNA) enable improved RNAi-based gene silencing

Citation summary: Comparison of cononical siRNA design to DsiRNAs for performance in siRNA processing and RISC assembly, leading to stronger silencing efficacy.

Read More>


Nanoparticle delivery of TNF-α targeting DsiRNA as treatment for rheumatoid arthritis

Citation summary: DsiRNA-loaded PLGA nanoparticles mediate dose-dependent silencing of TNF-α in vitro.

Read More>


In vivo delivery of aptamer–DsiRNA molecules targeting HIV-1

Citation summary: Learn how these scientists improve on in vivo siRNA delivery using a modified aptamer-DsiRNA (Dicer-substrate RNA; IDT) molecule to inhibit HIV-1 replication.

Read More>


Using antisense technologies to modulate noncoding RNA function

RNase H–dependent antisense silencing using gapmer ASOs is a powerful tool for specific modulation of nuclear noncoding RNAs. Review these useful modifications and design considerations for effective antisense oligonucleotides.

Read More>


Small RNA therapies for cystic fibrosis

The McCray Lab (Univ IA, USA) used DsiRNAs to develop a protocol for efficient small RNA delivery into airway epithelia. The resulting method was then used to study miRNA regulation of gene products involved in airway fluid and electrolyte transport, cellular mechanisms linked to cystic fibrosis.

Read More>


Towards real-time imaging in single, living cells

Learn how this research team is developing oligonucleotide technologies for detection and quantification of specific RNA transcripts in live cells using molecular beacons and gBlocks Gene Fragments..

Read More>


Inhibiting miRNAs using antisense oligonucleotides

Learn the basics about miRNA Inhibitors (also known as anti-miRNA oligos, or AMOs) for knockdown of miRNA expression in loss/gain of function studies.

Read More>


ReadyMade negative controls for gene silencing (RNAi) experiments

Obtain universal negative controls for DsiRNA transfections. These controls are premade, inexpensive, and ready to ship.

Read More>


RNAi and DsiRNA: Pathway, mechanism, and design

Read this overview of the RNA interference (RNAi) pathway. Learn about its applications and design considerations for gene silencing experiments.

Read More>


The future of RNAi and aptamer technologies

An interview with Dr John Rossi, Chair and Professor of the Department of Molecular and Cellular Biology at the Beckman Research Institute of City of Hope (Duarte, CA). His research focuses on the biology and applications of eukaryotic small RNAs and, in particular, their therapeutic use in HIV/AIDS and cancer.

Read More>



siRNAs with increased potency

Use IDT DsiRNAs to engage the Dicer-processing/RISC-loading mechanisms and get increased silencing potency over traditional siRNAs.

Learn more ≫


Related Articles

RNAi and DsiRNA: Pathway, Mechanism, and Design

An overview of the RNA interference (RNAi) pathway, its applications, and design considerations for gene silencing experiments.

Read more ≫

Using Antisense Technologies to Modulate Noncoding RNA Function

Useful modifications and design considerations for effective antisense oligonucleotides.

Read more ≫

Small RNA Therapies for Cystic Fibrosis

DsiRNAs are used to develop a protocol for efficient small RNA delivery into airway epithelia to study of miRNA regulation of airway fluid and electrolyte transport.

Read more ≫

Using DsiRNA to Map Pain Pathways in the CNS

Canadian laboratory targets pain receptors for expression knock out via in vivo delivery of DsiRNA 27mer duplex RNAs into the mouse CNS.

Read more ≫

The Future of RNAi and Aptamer Technologies—An Interview with John Rossi

An interview with Dr John Rossi, whose research focuses on the biology and applications of eukaryotic small RNAs and, in particular, their therapeutic use in HIV/AIDS and cancer.

Read more ≫