Small RNAs/Functional Genomics
Support and Educational Content

Nano-delivery of DsiRNAs results in improved gene silencing and anticancer activity

Liu X, Liu C, et al. (2014) Targeted delivery of Dicer-substrate siRNAs using a dual targeting peptide decorated dendrimer delivery system. Nanomedicine, 10(8):1627–1636.

Liu and colleagues have developed an siRNA delivery method that addresses 2 major challenges of using RNA interference for treating diseases: increasing siRNA potency and providing more targeted delivery. Working with
in vitro and in vivo model systems for prostate cancer, the scientists used a dendrimer-based, targeted nano-delivery system to transport Dicer Substrate RNAs (DsiRNAs) into PC-3 cells and nude mice bearing PC-3 prostate cancer xenografts. Coupling this delivery mechanism with DsiRNAs resulted in a much greater RNAi response and, thus, improved therapeutic efficacy, than when conventional siRNAs were used. Further decoration of the DsiRNA/dendrimer complexes with a peptide that simultaneously promotes cancer cell targeting and penetration, led to improved gene silencing and anticancer activity in prostate cancer models in vitro and in vivo.

Product focus—RNAi applications

Dicer-Substrate RNAs (DsiRNAs)

DsiRNA (Dicer-substrate RNAs) are chemically synthesized 27mer duplex RNAs that have increased potency in RNAi experiments compared to traditional siRNAs.

Learn more about DsiRNAs and order them from the DsiRNA product page.

TriFECTa® RNAi Kit

The TriFECTa RNAi Kit contains 3 Dicer-substrate 27mer duplexes, targeting a specific gene, that are selected from a predesigned set of duplexes from the RefSeq collection of human, mouse, and rat genes in Genbank.

Learn more from TriFECTa product page.

Secondary structure prediction software

The Unafold tool is free, online, secondary structure prediction software. The program calculates the probability of single-stranded RNA or DNA folding, or hybridization between 2 single strands.

Access the UNAFold tool here.

Additional DsiRNA resources:

DsiRNA articles—Learn more about applications of these siRNA-like 27mer duplex RNAs from other DECODED Online Newsletter articles.

Gene silencing webinars—Listen to presentations on siRNA and DsiRNA experimental design, as well as analysis and troubleshooting tips.

Selected DsiRNA citations—Scan through a list of peer-reviewed publications describing how your colleagues have successfully used IDT siRNA products and design tools in their research.

Author: Ellen Prediger, PhD, is a Scientific Writer at IDT.

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siRNAs with increased potency

Use IDT DsiRNAs to engage the Dicer-processing/RISC-loading mechanisms and get increased silencing potency over traditional siRNAs.

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