Synthetic Biology
Support and Educational Content

Plates of custom DNA fragments for high-throughput applications

gBlocks® Gene Fragments in Plates

gBlocks® Gene Fragments have been making synthetic biology research easier for many scientists. From qPCR controls to mutagenesis experiments to CRISPR/Cas9 genome editing, the double-stranded DNA fragments are becoming an indispensable tool in many labs. CRISPR/Cas9 genome editing is a particularly active research area where gBlocks fragments have proven very convenient. Esvelt KM, Mali P, et al (2013) [1] used the fragments to generate human optimized Cas9 orthologs from S thermophilusN meningitidis, and T denticola, as well as tracRNA expression cassettes for each ortholog (read a summary of their paper here).

To help accommodate this type of research where many DNA fragments are needed, IDT is providing gBlocks fragments in 96-well plates. Plates allow the researcher more options for large orders and high-throughput oligo needs.

With a minimum order of 48 sequences, gBlocks Gene Fragments are shipped suspended in 25 µL of nuclease-free water at 10 ng/µL. Plates are available for fragments of 200–1000 bp and are shipped within 10 days of order confirmation. (Longer gBlocks Gene Fragments—up to 3000 bp—can be ordered in tubes.)


gBlocks Gene Fragments are already helping researchers at the NIH:

"Using IDT’s synthetic DNA fragments (gBlocks Gene Fragments) has fundamentally transformed our approach to molecular cloning. What often used to be a time-consuming effort has now become a straightforward and predictable process. What’s more, gBlocks fragments are flexible and allow for precise tailoring of recombinant DNA constructs."

 – Dr Detlef Vulhord, Fellow Researcher, NIH/NICHD (Bethesda, MD, USA)

Learn how gBlocks Gene Fragments can speed up your research at www.idtdna.com/gblocks.  


Reference

  1. Esvelt KM, Mali P, et al. (2013) Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods, 10(11):1116–1121. doi: 10.1038/nmeth.2681.

 

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gBlocks® Gene Fragments

Double-stranded DNA up to 3,000 kb—great for easy gene construction, CRISPR genome editing, and more.

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