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Design efficient PCR and qPCR primers and probes using online tools

Simplify planning of your qPCR experiments using IDT free, online tools for oligonucleotide analysis and PCR primer design. This article provides an overview of our predesigned qPCR assays and the basics of designing customized PCR primers and hydrolysis probes with the PrimerQuest® Tool.

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PrimerQuest® Tool: From basic to highly customizable designs

Read these tips on how to use the PrimerQuest Tool to customize primers and probe for a wide variety of qPCR applications. Examples show how to adjust reaction conditions, add a probe to a set of previously designed primers, define primer positions, and include or exclude sequences from the assay designs.

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Selecting qPCR assays in plates for high throughput and large-scale studies

Learn some tricks for easy ordering of qPCR assays in plates.

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Codon optimization tool makes synthetic gene design easy

Use the free IDT Codon Optimization Tool to simplify designing synthetic genes and gBlocks® Gene Fragments for expression in a variety of organisms. The tool allows for manual changes, and takes into account natural codon bias and synthesis complexity.

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Easy resuspension and dilution of oligonucleotides

Save time by using these free resuspension and dilution calculators. A variety of units can be used as input values. Review the screen shots that show how these calculation tools can help you move on with your research.

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Determining the physical characteristics of your oligos—the OligoAnalyzer Tool

Use this free web tool to determine many of the physical characteristics of your oligonucleotides. By simply inputting your sequence, you can find out its length, GC content, melting temperature range, molecular weight, extinction coefficient, and optical density.

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Your Questions Answered by a Live Expert: IDT Web Chat Service

How to use the live chat feature on the IDT website.

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Personalized Group Ordering for Your Lab

Free IDT web tool platform that lets researchers consolidate orders, customize the lab ordering process, and share ideas and experimental findings.

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Making Ordering Easier

IDT offers three convenient methods of ordering.

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PrimeTime Predesigned qPCR Assays Selection Tool

IDT’s design engine incorporates many parameters to produce the best possible PrimeTime Pre-designed qPCR Assay.

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