Calculation tips for resuspending and diluting nucleic acids

Lab tips: Use these simple guidelines for making a 100 µM solution; calculating nanomoles, micrograms, copy number, and concentration; and determining concentration equivalencies.

Nov 4, 2012

Revised/updated Jan 23, 2017

Below are some of the common calculations we use when we work with oligonucleotides in our labs.

Making a 100 μM solution


To resuspend your oligonucleotides to 100 μM, simply multiply the number of nanomoles (nmol) by 10 to get the volume (in μL) of water or buffer to add. The equation on the left shows an example, assuming you have an oligonucleotide of 1 nmol final yield.

Calculating nmoles



Use this formula to calculate nmol when only the OD (absorbance at 260 nm) and extinction coefficient are provided.

Calculating micrograms



Use this formula to calculate micrograms (μg) of oligo when nmol and molecular weight (g/mol) are provided.


Calculating copy number


Use this formula to calculate the number of copies of your DNA sequence when moles are provided.

Calculating concentration


Use these formulas to calculate molar concentration (μM) when molecular weight (g/mol) and concentration in μg/μL are provided.

Access the free, online IDT Resuspension Calculator.