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Increase oligo stability with phosphorothioate modifications

Product spotlight: Need oligos that are nuclease resistant? Phosphorothioate bonds substitute a sulfur atom for one of the non-bridging oxygen atoms in the phosphate backbone of an oligonucleotide. Resistant to both endo- and exonucleases, this linkage provides increased oligo stability.

Sep 12, 2011

Quick facts:

Availability: DNA or RNA
Scales: 100 nmol-large scale
Purification: HPLC or PAGE
Symbol for ordering: * placed between 2 nucleotides

dcd13_CEmod

Figure 1. Phosphorothioate modification.

Preventing nuclease degradation

Phosphorothioate bonds substitute a sulfur atom for one of the non-bridging oxygen atoms in the phosphate backbone of an oligonucleotide. Phosphorothioates are primarily incorporated to prevent nuclease degradation. Substituting a sulfur atom for an oxygen atom renders the internucleotide linkage resistant to both endo- and exonucleases. Placing this modification between nucleotides at either terminus of an oligonucleotide sequence will provide exonuclease protection, while using the modification internally will inhibit endonuclease degradation. To order, place an asterisk (*) between 2 nucleotides, indicating the bond you would like to modify (e.g., A*CTG*ATCG*A).

Other modifications to facilitate your research

See what other modifications are available from IDT. You can find a list of standard modifications we make to oligonucleotides on the Modifications page of our online catalog. And if you don’t find what you are looking for, just send us a request. We often synthesize special, non-catalog requests. Contact us at noncat@idtdna.com.

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