LabReady Oligo Service
IDT will rehydrate your oligos at a fixed concentration prior to shipment as part of our LabReady Oligo Service. For a fee, your 25, 100, or 250 nmole scale oligos are shipped hydrated at 100 µM in IDTE (1X TE Buffer), pH 8.0.
Custom Normalized Tubes
For ultimate convenience, specify the concentration at which you would like to receive your oligos. Buffer choices are RNase-free water*, or IDTE (1X TE Buffer), pH 8.0 or 7.5. Applies to 25 nmole, 100 nmole, 250 nmole, and 1 µmole scales. For resuspension of larger scales, please contact customer service at firstname.lastname@example.org.
For a small mixing fee, have 2–6 standard desalt oligos premixed in a single tube according to your specifications. Oligos may contain modifications. Yields range between 5 and 50 nmole, and oligos can be shipped dried down or hydrated (in RNase-free water* or IDTE 8.0).
* To ensure longer stability of oligos, IDT recommends resuspension in TE.
IDT offers oligonucleotides containing randomized, or “mixed bases”. Mixed Bases are used in primers to bind to templates that contain variability or a mixture of sequences at the primer binding site. Mixed Bases can also be used to create diversity in clone libraries and in site-directed mutagenesis.
IDT offers two types of randomization, Machine Mixed and Hand Mixed Bases. Machine Mixed Bases can be made at any/all base sites at no additional charge. At these base positions, the synthesizer pulls an equal ratio of the desired bases, but their different coupling rates do not guarantee an equal ratio of incorporation.
Hand Mixed Bases provide customer-defined ratios of the desired bases, where coupling efficiencies are taken into account. For each mixture, IDT can incorporate up to 100 residues on the 100 nmole and 250 nmole scales and up to 40 residues on the 1 µmole scale, with a maximum of 4 unique Hand Mixes per oligonucleotide.When entering your sequence, use the “Mixed Base” tab at the bottom of the page. It lists the IUPAC-IUB symbols and is where custom mix ratios should be entered.
Additional Customized Formulation and Mixing Services
For aliquots or for more complex formulation or mixing needs, please contact customer service at email@example.com.