Ultramer Oligonucleotide Citations

  1. The insulator protein CTCF binding sites in the orf73/LANA promoter region of Herpesvirus Saimiri are involved in conferring episomal stability in latently infected human T cells
    Zielke K, Full F, et al
    J Virol, 86(3):1862–1873 (2012)

    Ultramer Oligonucleotides were used for BAC mutagenesis via a two-step l–red mediated recombination strategy.

  2. Development and validation of a semiquantitative, multitarget PCR Assay for diagnosis of bacterial vaginosis
    Cartwright CP, Lembke BD, et al
    J Clin Microbiol, 50(7):2321–2329 (2012)
    Ultramer Oligonucleotides used to construct calibration standards. Each Ultramer oligonucleotide contained a target sequence from one of the transcripts under study. All qPCR assay runs included a set of 3 Ultramer oligonucleotide calibrators.
  3. Pantethine rescues phosphopantothenoylcysteine synthetase and phosphopantothenoylcysteine decarboxylase deficiency in Escherichia coli but not in Pseudomonas aeruginosa
    Balibar CJ, Hollis-Symynkywicz MF, Tao J
    J Bacteriol, 193(13):3304–3312 (2011)

    Ultramer oligonucleotides were used to construct a new vector that provides one-step incorporation of the PBAD promoter in front of any gene.

  4. Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome
    Flynn RA, Almada AE, Zamudio JR, Sharp PA
    PNAS, 108(26):10460–10465 (2011)

    Ultramer Oligonucleotides used to generate standard curves for absolute quantification. Each Ultramer standard was designed to contain the 5′-end of a transcript under study and then qPCR amplified using transcript-specific primers.

  5. Strict Sex-Specific mtDNA Segregation in the Germ line of the DUI Species Venerupis philippinarum (Bivalvia: Veneridae)
    Ghiselli F, Milani L, Passamonti M
    Mol Biol Evol, 28(2):949–961 (2011)

    Ultramer Oligonucleotides used as standards, both due to their purity (not biologically derived; no  genomic DNA handling involved) and quantification precision (exact molecular weight known, so copy number calculation is more precise).

  6. miR-212 increases tumor necrosis factor–related apoptosis-inducing ligand sensitivity in non–small cell lung cancer by targeting the antiapoptotic protein PED
    Incoronato M, Garofalo M, et al
    Cancer Res, 70(9):3638–3646 (2010)

    Use of the in situ detection of mature microRNAs by labeled extension on ultramer templates method described by Nuovo et al [see 2009 Nuovo reference, below] to detect human miR-212 expression patterns.

  7. Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
    Gibson D
    Nucleic Acids Res, 37(20):6984–6990 (2009)

    Use of sets of hybridized, high-fidelity Ultramer Oligonucleotides to assemble large constructs in a single step. Now available as a product, gBlocks® Gene Fragments, with the high-fidelity Ultramer oligos verified and prehybridized, ready for assembly.