Related DECODED Articles

Guaranteed knockdown of RNA in loss-of-function studies

Product spotlight: Predesigned DsiRNAs in the TriFECTa Kits are guaranteed to knockdown gene expression in RNA interference (RNAi) studies.

Read More>

A new renaissance for antisense in the era of lncRNA

Noncoding RNAs such as lncRNA, are much more prevalent in humans than protein-coding RNA. Antisense oligonucleotides (ASO), previously used for knockout experiments, are being employed to study the role of noncoding RNAs in gene regulation. ASOs provide several advantages over siRNAs (and DsiRNA) for this purpose.

Read More>

Nano-delivery of DsiRNAs results in improved gene silencing and anticancer activity

Citation summary: Find out how a dendrimer-based, targeted nano-delivery system that uses Dicer Substrate RNAs (DsiRNAs) leads to improved gene silencing and anticancer activity in prostate cancer models in vitro and in vivo.

Read More>

Using DsiRNA to map pain pathways in the CNS

DsiRNAs (Dicer-substrate RNAs) are chemically synthesized 27mer duplex RNAs that have increased potency in RNA interference compared to traditional 21mer siRNAs. Read this example of a research group using the technology for in vivo delivery to the CNS.

Read More>

Advantageous properties of Dicer-Substrate Interfering RNA (DsiRNA) enable improved RNAi-based gene silencing

Citation summary: Comparison of cononical siRNA design to DsiRNAs for performance in siRNA processing and RISC assembly, leading to stronger silencing efficacy.

Read More>

In vivo delivery of aptamer–DsiRNA molecules targeting HIV-1

Citation summary: Learn how these scientists improve on in vivo siRNA delivery using a modified aptamer-DsiRNA (Dicer-substrate RNA; IDT) molecule to inhibit HIV-1 replication.

Read More>

Small RNA therapies for cystic fibrosis

The McCray Lab (Univ IA, USA) used DsiRNAs to develop a protocol for efficient small RNA delivery into airway epithelia. The resulting method was then used to study miRNA regulation of gene products involved in airway fluid and electrolyte transport, cellular mechanisms linked to cystic fibrosis.

Read More>

RNAi and DsiRNA: Pathway, mechanism, and design

Read this overview of the RNA interference (RNAi) pathway. Learn about its applications and design considerations for gene silencing experiments.

Read More>

The future of RNAi and aptamer technologies

An interview with Dr John Rossi, Chair and Professor of the Department of Molecular and Cellular Biology at the Beckman Research Institute of City of Hope (Duarte, CA). His research focuses on the biology and applications of eukaryotic small RNAs and, in particular, their therapeutic use in HIV/AIDS and cancer.

Read More>