PrimeTime® Assays with Fast-Cycling Protocols—Comparative Data

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Comparison of NM_001182 Assay Performance Comparison of NM_003105 Assay Performance Comparison of NM_001855 Assay Performance Comparison of NM_000671 Assay Performance Comparison of NM_000362 Assay Performance Comparison of NM_003596 Assay Performance Comparison of NM_002581 Assay Performance Comparison of NM_004308 Assay Performance Comparison of NM_005188 Assay Performance Comparison of NM_005768 Assay Performance Comparison of NM_006325 Assay Performance Comparison of NM_003922 Assay Performance Comparison of NM_006500 Assay Performance Comparison of NM_004343 Assay Performance Comparison of NM_005385 Assay Performance Comparison of NM_006784 Assay Performance Comparison of NM_007024 Assay Performance Comparison of NM_012204 Assay Performance Comparison of NM_014109 Assay Performance Comparison of NM_014155 Assay Performance Comparison of NM_006782 Assay Performance Comparison of NM_014367 Assay Performance Comparison of NM_005911 Assay Performance Comparison of NM_014630 Assay Performance Comparison of NM_014742 Assay Performance
   
  WDR3 TIMP3 HERC1
Mean Cq (50 ng) PrimeTime® 22.9 23.9 25.1
Competitor A 24.0 24.1 26.2
Efficiency PrimeTime® 97.0% 95.0% 95.9%
Competitor A 96.4% 91.7% 86.8%
Endpoint (ΔRn) PrimeTime® 10.9 8.9 7.9
Competitor A 7.2 6.1 7.0

PrimeTime® qPCR Assays Maintain Efficiency Under Fast-Cycling Conditions. 25 PrimeTime qPCR Assays (blue curves) were compared to equivalent Competitor A assays (consisting of 15 inventoried pre-designed assays and 10 made-to-order pre-designed assays; red curves). To ensure an accurate comparison was made, the PrimeTime Assays and Competitor A assays were selected to span the same exon boundary of each gene. Reactions included five 4-fold dilutions cDNA template and the Applied Biosystems TaqMan® Gene Expression Master Mix, and were run on the ABI 7900HT Fast Real-Time PCR System with the following PCR cycling conditions: 2 min. 50°C; 10 min. 95°C; 45 x (15 sec. 95°C, 1 min. 60°C). Identical thresholds were set for all runs for comparison across assays.