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Review these definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments.

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A conversation about qPCR with Jo Vandesompele

Research profile: An interview with Dr Jo Vandesompele, internationally recognized expert on quantitative PCR, discussing the common issues researchers face when using qPCR, and the future direction of qPCR technology.

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Sample preparation for successful qPCR

Read about qPCR sample preparation and what experimental details you should consider for obtaining accurate and consistent results.

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Steps for a successful qPCR experiment

Read these recommendations for 5′ nuclease assay design and experimental setup that will help you obtain accurate and consistent results.

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Identifying stem cell differentiation biomarkers

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Using qPCR assays in multiplex experiments

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PrimeTime Predesigned qPCR Assays Selection Tool

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Tips for using BLAST to locate PCR primers

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