Related DECODED Articles

Do Your qPCR Assays Come With Sequence Information? They Should. Here Is Why.

qPCR assays (primer & probe sets) from other suppliers are often provided without sequence information. IDT always gives you the sequences to the oligos you order. And that can be very important. Read why.

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Modification Highlight: ZEN™ Internal Quencher

Use of the ZEN Quencher as a second, internal quencher in qPCR 5’-nuclease assay probes provides greater overall dye quenching, lowering background, and increasing signal detection. When incorporated into oligonucleotides, it also serves to strengthen duplex formation and block exonuclease digestion, while remaining nontoxic to cells. Thus the ZEN Quencher can be useful in steric blocking antisense oligonucleotide applications.

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Multiplex qPCR—How to Get Started

Learn how multiplex qPCR can save sample, reagent cost, and time. The article provides recommendations for multiplex qPCR assay design and experimental setup.

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qPCR Terminology—What Does It Mean?

Definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments.

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qPCR with Intercalating Dyes: PrimeTime® qPCR Primers

PrimeTime qPCR Primers are now available to detect genes in human, mouse, and rat transcriptomes with intercalating dyes such as SYBR® Green and EvaGreen®

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A Conversation About qPCR with Jo Vandesompele

An interview with Dr Jo Vandesompele, internationally recognized expert on quantitative PCR, discussing the common issues researchers face when using qPCR, and the future direction of qPCR technology.

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Sample Preparation for Successful qPCR

Considerations for qPCR sample preparation for obtaining accurate and consistent results.

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Steps for a Successful qPCR Experiment

This article will focus on 5′ nuclease assay design and experimental setup considerations that will assist in obtaining accurate and consistent results.

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Up-to-Date Assay Design—Avoiding SNPs

Keeping up to date with the continually increasing number of annotated SNPs is critical when designing qPCR assays, particularly when working with samples that may have different or unknown genetic backgrounds.

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Identifying Stem Cell Differentiation Biomarkers

MultiStem have greater differentiation potential than typical mesenchymal stem cells.

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Choosing a qPCR Assay: Inventoried or Predesigned?

Several companies offer inventoried qPCR assays that have not only been predesigned, but are also premanufactured and stocked, waiting for that order to be placed. In contrast, IDT PrimeTime Pre-designed qPCR Assays are not pre-manufactured and have undergone rigorous bioinformatics assessments using sophisticated design algorithms to generate the most efficient assays for a particular genetic region.

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Using PrimeTime qPCR Assays in Multiplex Experiments

IDT PrimeTime qPCR Assays offer multiple dye/quencher combinations and primer/probe ratios to simplify the multiplex experiment design.

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PrimeTime Predesigned qPCR Assays Selection Tool

IDT’s design engine incorporates many parameters to produce the best possible PrimeTime Pre-designed qPCR Assay.

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Tips for Using BLAST to Locate PCR Primers

Need a quick way to find the location of primers within a gene or the expected size of the resultant PCR product? In this tip we show you how to get this information using BLAST.

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