Targeted CRISPR/Cas9 Genome Editing

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The clustered regularly interspaced short palindromic repeat (CRISPR) system is an RNA guided genome editing tool that is revolutionizing genome engineering.

Unlike the older TALEN and zinc-finger nuclease methods, CRISPR does not require labs to be experts at protein engineering to target specific sites in the genome. With the CRISPR system, you can generate or purchase one Cas9 endonuclease for your specific organism or cell type, and target many sites by changing the short, 20 bp recognition sequence of the single-guide RNA. The result is a system that relatively easy to implement, and highly flexible compared to older methods.