gBlocks® Gene Fragments—Related DECODED Articles


Tips for working with gBlocks® Gene Fragments

Working with IDT custom, synthetic dsDNA fragments? Get these tips from our scientists on the best ways to resuspend, quantify, and calculate copy number of gBlocks® Gene Fragments.

Read More>


Pakistani iGEM team’s biosensor detects vehicle emission levels

Research profile: Learn how the first ever Pakistani iGEM team developed a portable, inexpensive vehicle emissions test to address air pollution. They used gBlocks Gene Fragments for construction of genetic circuits to detect carbon monoxide and nitrogen oxides. The colorimetric biosensor won this young research team a bronze medal at the 2016 iGEM International Jamboree.

Read More>


iGEM, and the vision for the next generation of synthetic biology researchers

Randy Rettberg, Co-founder and President of iGEM, discusses the 2016 iGEM competition in this video interview. Learn about his vision for the future for the students that participate in iGEM, as well as the future of synthetic biology.

Read More>


Zika virus: Advances in disease modeling and detection

IDT is supporting global research aimed at reducing the widespread effects of Zika. Learn about the virus, and read a summary of the latest developments.

Read More>


RNA-guided gene drives for inheritance bias in yeast: Safe, responsible genome editing

Citation summary: Read about how a group of researchers use gBlocks® Gene Fragments and novel precautionary measures to responsibly investigate Cas9-based eukaryotic inheritance bias of gene drives.

Read More>


Czech Republic iGEM team’s diagnostic for circulating tumor cells

Research profile: Read how students participating on the Czech Republic’s first iGEM team reprogrammed yeast cells to identify circulating tumor cells. Their project, the IOD Band, could become a general diagnostic test for early detection and mapping of tumor cell mobility. IDT gBlocks® Gene Fragments facilitated rapid, construct assembly of IOD Band receptor molecules.

Read More>


Towards next generation biosensors

Research profile: Read how fluorescent protein-based Ca2+ indicators based on naturally occurring substrates are assembled using gBlocks Gene Fragments. These sensors are being developed to monitor in vivo neural activity.

Read More>


Site-directed mutagenesis—improvements to established methods

Site-directed mutagenesis techniques have relied primarily on PCR and standard cloning methods. Read about some of the common cloning methods used for mutagenesis and how double-stranded DNA fragments (gBlocks Gene Fragments) can save you both time and money.

Read More>


Overlapping biocontainment strategies for genetically modified E. coli

Citation summary: gBlocks Gene Fragments are used to create codon optimized components of a biocontainment system for genetically modified E. coli.

Read More>


iGEM Students Engineer Biological Tools for a Better World

Projects from 2 of the prize-winning 2013 iGEM teams show how non-standard natural and synthetic amino acids can be used in 1) peptide synthesis, and 2) tuberculosis monitoring and treatment. Both projects make use of gBlocks® Gene Fragments to speed construct assembly.

Read More>


The gene construction revolution

See how use of high-quality, custom dsDNA fragments as a starting material allows you to turn what might otherwise be multi-step cloning assemblies into simpler reactions.You can often just order the entire target sequence ready for cloning or other uses.

Read More>


Creating a synthetic immune system for optimized immune profiling

Research profile: A high-throughput method for sequencing and quantification of rearranged antigen receptors on T- and B-cells using gBlocks Gene Fragments to create a “gold standard synthetic immune system” where the binding sites for every possible forward and reverse primer combination for a given receptor type were represented.

Read More>


CRISPR and Cas9 for flexible genome editing

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) are sequences that serve as an adaptive defense ("immune") systems in bacteria and archaea. Learn how scientists have coopted this natural mechanism for targeted gene editing or removal. This article also describes some of the early applications for which this technology is being used.

Read More>


A high throughput, high resolution melting protocol for identifying single-nucleotide polymorphisms

Citation summary: Rapid SNP evaluation by HTP melt-curve analysis, using gBlocks® Gene Fragments as melt-curve controls.

Read More>


Caffeine addicted bacteria

Citation summary: Use of IDT gBlocks® Gene Fragments in Gibson Assembly® reactions to generate some of the plasmid constructs in this work.

Read More>


Innovative Therapeutic Antibodies

Use of gBlocks® Gene Fragments to synthesize antibody variable regions to create novel heavy/light chain combinations.

Read More>


Easily designed standard curves for qPCR

Adopt this easy way to combine control templates/multiple targets onto a single construct, and get the advantages that they provide for PCR experiments.

Read More>


DNA methylation analysis—keeping it simple

Research profile: Use of methylation-sensitive restriction enzymes and probe-based PCR to provide methylation percentage for specific amplicon regions (primarily promoters). Read how IDT ZEN Double-Quenched Probes, PrimeTime qPCR Assays, and gBlocks Gene Fragments augment this analysis.

Read More>


Predictable control of gene expression by mRNA, 3’ untranslated region motifs

Citation summary: See how these researchers use gBlocks® Gene Fragments as qPCR standards to generate DNA standard curves for absolute quantification of mRNA.

Read More>


Small RNA regulation of guanine quartet formation and antigenic variation

Citation summary: Using gBlocks® Gene Fragments, these researchers create mutated version of a small RNA to show how it facilitates the formation of a guanine quartet.

Read More>