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Genotyping

IDT offers easy-to-use genotyping components for the identification of SNPs, CNVs, indels, and more.

rhAmp™ SNP Genotyping System

The rhAmp SNP Genotyping System is a fully integrated genotyping solution that includes an extensive predesigned sequence collection, a custom design tool, reagent mixes, and optional synthetic control templates. SNP identification may be performed on any commonly available qPCR instrument.

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Affinity Plus™ qPCR Probes

Use Affinity Plus qPCR Probes for enhanced discrimination of thermodynamically similar samples such as single nucleotide polymorphisms and transcript variants. The Affinity Plus bases used in these qPCR probes include up to 6 locked nucleic acid monomers. When incorporated into a probe, locked nucleic acids impart heightened structural stability, leading to increased hybridization melt temperature (Tm).

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PrimeTime™ LNA® qPCR Probes

PrimeTime LNA qPCR probe sequence (10–25 standard bases with up to 6 locked nucleic acid nucleotides), reporter, and quencher. Choose from a wide variety of reporter and quencher combinations.

LNA is a registered trademark of Qiagen.

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MGB Eclipse® Probes

MGB Eclipse probes and companion primers are manufactured under ISO 13485 conditions, which can help you transition from research to clinical diagnostics.

MGB Eclipse is a registered trademark of ELITech Group.

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PACE™ SNP Genotyping Assays

PACE (PCR Allele Competitive Extension) SNP genotyping assays are well-suited for projects that require a reliable, cost-effective approach for SNP and indel identification.

PACE is a trademark of 3CR Bioscience

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Overview

Researchers often look for genetic variations to better understand phenotypic traits, such as plant fruit production. One individual's genome may differ from others in numerous ways, including single nucleotide polymorphisms (SNPs), insertions or deletions (indels), or copy number variations (CNV).

Researchers identify these differences using a variety of techniques such as polymerase chain reaction (PCR) and quantitative PCR (qPCR). The latter of which often employs a primer-pair and fluorescent probes designed to target a gene and to help identify SNPs. IDT offers predesigned assays, as well as complementary easy- and ready-to-use reagent mixes for SNP identification. For other applications, modified probes are available that can be incorporated into custom qPCR assays.

Resources

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