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Focused sequencing using target capture probes helps identify a post-fertilization mutation in BRCA1 that may predict risk of early-onset breast cancer.

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Towards Providing Personalized Medicine—Considerations for Reliable NGS Data

Scientists at Geneseeq Technology, Inc. demonstrate how they improved their target capture methods to increase accuracy in clinical diagnostics by using optimized blocking oligos and stringent hybridization conditions.

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Next Generation Sequencing in the Clinic: A Perspective from Dr Elaine Mardis

Dr Elaine Mardis shares her views on the current uses of NGS, the challenges that NGS technologies face, and what can be expected in the future.

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Reduced Barcode Contamination using Oligonucleotides with TruGrade™ Processing

An NGS facility, GenePool, notes how barcode crosstalk can severely affect next generation sequencing results and how TruGrade processing can obviate that.

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Genomic Target Selection Using Individually Synthesized Capture Probes

Foundation Medicine, Inc describes development of its cancer diagnostic genomic test for solid tumors, with the goal to provide a fully informative profile that helps physicians make personalized therapy decisions for patients with cancer. It has been optimized for FFPE samples and small specimens and interrogates over 182 cancer-related genes plus 37 introns from 14 genes frequently rearranged in cancer. Their research shows how IDT Biotinylated Ultramer™ Oligonucleotides perform better than array-synthesized probes during target capture for next generation sequencing with this panel.

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