xGen Lockdown Probes citations

  1. Circulating tumor DNA mutation profiling by targeted next generation sequencing provides guidance for personalized treatments in multiple cancer types
    Shu Y, Wu X, et al
    Sci Rep, 7(1):583 (2017)

    xGen Lockdown Probes and Blocking Oligos were used to generate a custom gene panel that targeted 382 cancer-relevant genes in multiple cancer types. The panel provided target enrichment across 605 circulating tumor (ctDNA) samples. 

  2. ve-SEQ: Robust, unbiased enrichment for streamlined detection and whole-genome sequencing of HCV and other highly diverse pathogens
    Bonsall D, Ansari MA, et al
    F1000 Research, 4:1062 (2015)

    Scientists at the University of Oxford used xGen® Lockdown® Probes in a new, high-throughput method for detecting and sequencing HCV genotypes.

  3. A systematic comparison of traditional and multigene panel testing for hereditary breast and ovarian cancer genes in more than 1000 patients
    Lincoln SE, Kobayashi Y, et al
    J Mol Diagn., 17(5):533–544 (2015)
    xGen® Lockdown® Probes were used to rescue drop-out regions of SureSelect® probe panels (Agilent), giving more uniform target coverage. The probes were able to target 29 genes from whole blood genomic DNA, isolated from over 1000 patients.
  4. Sequencing small genomic targets with high efficiency and extreme accuracy
    Schmitt MW, Fox EJ, et al
    Nat Methods, 12(5):423–425 (2015)

    xGen Lockdown Probes were used to target regions of the genome containing commonly mutated genes. The probes allowed the authors to achieve sequencing with more specificity and depth, which greatly improved the efficiency and cost of data collection.  

  5. Low-level constitutional mosaicism of a de novo BRCA1 gene mutation
    E Friedman, N Efrat, et al
    Br J Cancer., 112(4):765–768 (2015)
    Next generation sequencing was used to accurately detect constitutional mosaicism in an individual diagnosed with early onset, high-grade breast cancer. xGen® Lockdown® Probes were used to target multiple genes of interest, including BRCA1, in DNA isolated from the individual’s blood.
  6. Prostate cancer risk locus at 8q24 as a regulatory hub by physical interactions with multiple genomic loci across the genome
    Du M, Yuan T, et al.
    Hum Mol Genet., 24(1):154–166 (2015)
    xGen® Lockdown® Probes were designed to target and capture segments of DNA at the 8q24 locus, a chromosomal region known for being involved in prostate cancer susceptibility.
  7. High-throughput sequencing in mitochondrial DNA research
    Fei Ye, David C. Samuels, et al
    Mitochondrion, 17:157–160 (2014)

    The authors explain how next generation sequencing has advanced mitochondrial genetics research. They note how IDT’s xGen Lockdown Probes can be used to accomplish solution phase capture of mtDNA. 

  8. Clinical evaluation of a multiple-gene sequencing panel for hereditary cancer risk assessment
    Kurian AW, Hare EE, et al
    J Clin Oncol., 32(19):2001–2009 (2014)
    xGen® Lockdown® Probes were used to target an entire coding region, exon-intron boundaries, and segments of DNA containing known pathogenic variants in BRCA1 and BRCA2 genes.
  9. ChildSeq-RNA: A next-generation sequencing-based diagnostic assay to identify known fusion transcripts in childhood sarcomas
    Qadir MA, Zhan SH, et al
    J Mol Diagn., 16(3):361–370 (2014)
    Target-capture using xGen® Lockdown® Probes was combined with throughput RNA sequencing to provide high resolution detection of known gene fusions. The targeted gene fusions were known to be associated with several childhood sarcomas.