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PAGE and HPLC Purification

Purification is recommended for all oligos greater than 40 bases in length and for many modified oligos. For demanding applications such as multiplex PCR, cloning, mutagenesis or antisense/RNAi methods, additional purification will significantly improve oligonucleotide performance. Every purified oligo up to 60 bases in length receives QC by capillary electrophoresis (CE) to document final purity.

Documentation and Support

Description100 nmole DNA oligo250 nmole DNA oligo1 µmole DNA oligo5 µmole DNA oligo10 µmole DNA oligo
PAGE Purification$45.00 USD$69.00 USD$115.00 USD$675.00 USD$995.00 USD
HPLC Purification$42.00 USD$65.00 USD$105.00 USD$240.00 USD$370.00 USD
Dual PAGE & HPLC PurificationInquire for Pricing$130.00 USD$240.00 USD$940.00 USD$1,450.00 USD
IE HPLC Purification$42.00 USD$65.00 USD$105.00 USD$240.00 USD$370.00 USD
RNase Free HPLC Purification$75.00 USD$105.00 USD$160.00 USD$340.00 USD$590.00 USD
Dual HPLC Purification$80.00 USD$120.00 USD$190.00 USD$500.00 USD$760.00 USD
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Guaranteed yields for purified, unmodified oligos 20 to 50 bases in length. Purity and yield guarantees may vary due to oligo length, modifications, or sequence composition.

  100 nmole 250 nmole 1 µmole 5 µmole 10 µmole
PAGE Purification
 1 OD 2 ODs 10 ODs 50 ODs 100 ODs
HPLC Purification
 1 OD
 4 ODs 20 ODs 100 ODs 200 ODs
IE-HPLC Purification  1 OD
 4 ODs 20 ODs 100 ODs 200 ODs
RNase-Free HPLC Purification
 1 OD
 4 ODs 20 ODs 100 ODs 200 ODs
Dual HPLC Purification
 1 OD
 2 ODs 10 ODs 50 ODs 100 ODs
Dual PAGE & HPLC
 0.5 OD 1 OD 5 ODs 25 ODs 50 ODs

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PAGE obtains extremely high purity, especially for unmodified oligos and Ultramer™ oligos. PAGE purification is strongly recommended for oligos over 60 bases in length. For unmodified oligos, purity of >90% is routinely achieved. Purity may vary due to oligo length, modifications, or sequence composition.

PAGE Purification

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HPLC is well suited to purify any modified oligo or unmodified sequences up to 60 bases in length. Purity of > 85% is guaranteed. Purity may vary due to oligo length, modifications, or sequence composition.

Ion-exchange HPLC (IE-HPLC) is available to purify long DNA oligos. For unmodified oligos, purity of > 85% is guaranteed. Purity may vary due to oligo length, modifications, or sequence composition.

For the most demanding applications, IDT offers Dual HPLC Purification and Dual PAGE and HPLC Purification. These methods result in oligos of the highest possible purity. Dual HPLC purification is used to purify Dual‑Labeled Probes and Molecular Beacons with NHS Ester modifications. Purity may vary due to oligo length, modifications, or sequence composition.

HPLC Purification

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Originally developed for isolating RNA oligos, RNase-Free HPLC Purification has been extended to DNA oligos for use when there is a high sensitivity to ribonucleases. RNase-Free HPLC purification is carefully performed in an RNase‑free environment; reagents, equipment and lab surfaces are monitored for RNase contamination using the RNaseAlert™ system.

Guaranteed yields for purified, unmodified oligos 20 to 50 bases in length. Purity and yield guarantees may vary due to oligo length, modifications, or sequence composition.

RNase-Free HPLC Purification

IDT Guarantees Quality

IDT has pioneered the use of high throughput Quality Control methods and is the only oligonucleotide manufacturer that offers 100% QC and purity guarantees. Today, every oligo synthesized at IDT undergoes QC by mass spectrometry. IDT also offers CE and analytical HPLC as routine tools for additional QC on purified oligos. In addition to DNA, IDT has led the effort over the last decade to reduce the cost of high-quality custom RNA synthesis. Through improvements to the traditional tert-butyldimethylsilyl (tBDMS) chemistry and advances in instrumentation, IDT has achieved the highest coupling efficiency in the industry. Only at IDT are customers guaranteed the very best in quality synthesis with free mass spectrometry and CE traces on all purified oligos.

 
 
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