Microbial identification assays

qPCR assays for rapid, sensitive microbial detection

Save time spent designing primers and probes by using predesigned assay sets. The following assays have been tested on the cobas omni Utility Channel:

C. difficile
Influenza A, B /RSV
HSV / VZV

Primers and probes are HPLC purified and verified by mass spectrometry, delivering batch-to-batch consistency to provide confidence in your data.

Product details

The primers and probes were tested using the Roche cobas omni Utility Channel Tool v3.0 and the cobas omni Utility Channel Reagent Kit. The parameters shown in Tables 1 and 2 were used.

Table 1.

	C. difficile	HSV-1 / HSV-2 / VZV	Influenza A / Influenza B / RSV
Channel number	Channel 2 (C. diff)	Channel 2 (HSV-1) Channel 4 (HSV-2) Channel 3 (VZV)	Channel 2 (Influenza A) Channel 3 (Influenza B) Channel 4 (RSV)
Primer/probe set volume	480 µL	480 µL	480 µL
MMx-2	8 mL	8 mL	8 mL
7 mL of the primer-probe and MMx-2 mix transferred to the cobas omni Utility Channel Reagent cassette
Sample type selected	Simple sample	Simple sample	Swab sample
Sample input volume	200 µL	200 µL (CSF) 500 µL (plasma and swab)	400 µL
RFI min	1.5 (C. diff)	1.6 (HSV-1) 1.5 (HSV-2) 1.8 (VZV)	1.3 (Influenza A) 2 (Influenza B) 1.2 (RSV)

Table 2. Cycling conditions.*

	UNG incubation	Pre-PCR step	1st measurement	2nd measurement	Cooling
No. of cycles	Predefined	1	5	45	Predefined
No. of steps		3	2	2	Predefined
Temperature		55°C; 60°C; 65°C	95°C; 55°C	91°C; 58°C
Hold time		120s; 360s; 240s	5s; 30s	5s; 25s
Data acquisition		None	End of each cycle	End of each cycle

* Default PCR profile for the cobas omni Utility Channel provided by the Utility Channel software.

Legal and regulatory information

This product and its use is the subject of one or more U.S. patent, and/or other pending U.S. and foreign patent applications owned by IDT. This includes patents and patent applications of IDT related to the right to use oligonucleotide probes labeled with Iowa Black FQ^®, Iowa Black RQ^®, TAO™, and/or ZEN™ quenchers. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and diagnostics for human healthcare conducted by the buyer. The buyer cannot sell or otherwise transfer this product or its components for a third party.