Labeled probes should, for the most part, be handled as other custom oligonucleotide sequences. We recommend resuspending probes in TE buffer (10 mM Tris pH 8.0, 0.1 mM EDTA), such as or IDTE
. Non-DEPC treated nuclease‑free water can be used, although it can limit shelf life. Ideally, for each resuspended probe, create a stock and several aliquots and store them all at –20°C. For long term oligo storage, temperature is the most important factor to consider. For long term storage, whether oligos are dried down, or resuspended in water (non-DEPC treated) or TE buffer, it is optimal to store them frozen.
For room temperature or 4°C storage, oligos that are resuspended in TE buffer are more stable than dry oligos. Oligos that are stored in water are the least stable. At 4°C oligos stored under all of these conditions are stable for at least 60 weeks.
Please see the Technical Report, Oligonucleotide Stability Study
for data on oligonucleotide storage and a more thorough explanation.