Frequently asked questions

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Can gBlocks® Gene Fragments be used for microinjection as sgRNAs for CRISPR applications?

A recent report compared the efficiency of microinjection of DNA versus RNA in mouse embryos [1]. While it was shown that DNA is effective, in vitro transcribed RNA was observed to be more efficient.
Typically, microinjections for CRISPR applications are performed using in vitro transcribed Cas9 and sgRNA rather than native dsDNA. gBlocks® Gene Fragments are ideal for use as template for in vitro transcription and will work well in these applications [2, 3].
  1. Horii T, Arai Y, et al. (2014) Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering. Scientific Reports,4:4513.
  2. Niu Y, Shen B, et al. (2014) Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell, 156(4):836–843.
  3. Wang H, Yang H, et al. (2013) One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell, 153(4):910–918.